Assay Detail
Binding
CHEMBL4394780
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of purified Escherichia coli BL21 (DE3) leuRS incubated for 10 mins in presence of 14C-labeled leucine by radiolabel transfer assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Escherichia coli BL21(DE3) |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Target
Leucine--tRNA ligase (CHEMBL4295566) ↗| Type | SINGLE PROTEIN |
| Organism | Escherichia coli (strain K12) |
Publication
Comparative analysis of pyrimidine substituted aminoacyl-sulfamoyl nucleosides as potential inhibitors targeting class I aminoacyl-tRNA synthetases.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 3 | 7.74 | 8.01 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4465674 | — | — | 1 | 8.01 |
| CHEMBL4467328 | — | — | 1 | 7.68 |
| CHEMBL4451258 | — | — | 1 | 7.53 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4465674 | — | Ki | = | 9.8 | nM | 8.01 |
| CHEMBL4467328 | — | Ki | = | 20.8 | nM | 7.68 |
| CHEMBL4451258 | — | Ki | = | 29.5 | nM | 7.53 |