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Assay Detail

CHEMBL4406743

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of (sCy5)-[Lys2 Arg4]-BVD15 from GFP-tagged Y1R in human HEK293T cells assessed as inhibitory constant incubated for 5 mins followed by (sCy5)-[Lys2 Arg4]-BVD15 addition and measured after 30 mins by fluorescence based assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Neuropeptide Y receptor type 1 (CHEMBL4777)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Heterodimeric Analogues of the Potent Y1R Antagonist 1229U91, Lacking One of the Pharmacophoric C-Terminal Structures, Retain Potent Y1R Affinity and Show Improved Selectivity over Y4R.
Richardson RR, Groenen M, Liu M, Mountford SJ, Briddon SJ, Holliday ND, Thompson PE.
J Med Chem (2020) 63 : 5274 -5286
PubMed 32364733 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 11 8.57 9.59

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4454036 1 9.59
CHEMBL4476074 1 9.56
CHEMBL4445317 1 9.44
CHEMBL4519035 1 9.24
CHEMBL2110365 1 8.99
CHEMBL4573545 1 8.63
CHEMBL4534028 1 8.09
CHEMBL4578617 1 7.89
CHEMBL267633 NEUROPEPTIDE-Y 1 7.78
CHEMBL4468261 1 7.60
CHEMBL4572358 1 7.50

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4454036 Ki = 0.257 nM 9.59
CHEMBL4476074 Ki = 0.2754 nM 9.56
CHEMBL4445317 Ki = 0.3631 nM 9.44
CHEMBL4519035 Ki = 0.5754 nM 9.24
CHEMBL2110365 Ki = 1.023 nM 8.99
CHEMBL4573545 Ki = 2.344 nM 8.63
CHEMBL4534028 Ki = 8.128 nM 8.09
CHEMBL4578617 Ki = 12.88 nM 7.89
CHEMBL267633 NEUROPEPTIDE-Y Ki = 16.6 nM 7.78
CHEMBL4468261 Ki = 25.12 nM 7.60
CHEMBL4572358 Ki = 31.62 nM 7.50