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Assay Detail

CHEMBL4409804

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of porcine liver carboxylesterase using 4-NPA as substrate preincubated for 10 mins followed by substrate addition by spectrophotometric analysis
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Sus scrofa
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Liver carboxylesterase (CHEMBL3383)
Type SINGLE PROTEIN
Organism Sus scrofa

Publication

Synthesis of 2-arylhydrazinylidene-3-oxo-4,4,4-trifluorobutanoic acids as new selective carboxylesterase inhibitors and radical scavengers.
Khudina OG, Makhaeva GF, Elkina NA, Boltneva NP, Serebryakova OG, Shchegolkov EV, Rudakova EV, Lushchekina SV, Burgart YV, Bachurin SO, Richardson RJ, Saloutin VI.
Bioorg Med Chem Lett (2019) 29 : 126716 -126716
PubMed 31640885 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 6.12 7.16

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4564380 1 7.16
CHEMBL4585339 1 6.49
CHEMBL4475685 1 6.20
CHEMBL1231178 1 5.75
CHEMBL4438172 1 5.58
CHEMBL4461638 1 5.56
CHEMBL153 TROLOX 1 -
CHEMBL196 ASCORBIC ACID 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4564380 IC50 = 69.6 nM 7.16
CHEMBL4585339 IC50 = 324.0 nM 6.49
CHEMBL4475685 IC50 = 630.0 nM 6.20
CHEMBL1231178 IC50 = 1800.0 nM 5.75
CHEMBL4438172 IC50 = 2620.0 nM 5.58
CHEMBL4461638 IC50 = 2760.0 nM 5.56
CHEMBL153 TROLOX IC50 - -
CHEMBL196 ASCORBIC ACID IC50 - -