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Assay Detail

CHEMBL4410836

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Inhibition of human recombinant CYP3A4 using midazolam as substrate incubated for 40 to 130 mins in presence of NADPH by LC-MS/MS analysis
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cytochrome P450 3A4 (CHEMBL340)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Scaffold Morphing Identifies 3-Pyridyl Azetidine Ureas as Inhibitors of Nicotinamide Phosphoribosyltransferase (NAMPT).
Palacios DS, Meredith EL, Kawanami T, Adams CM, Chen X, Darsigny V, Palermo M, Baird D, George EL, Guy C, Hewett J, Tierney L, Thigale S, Wang L, Weihofen WA.
ACS Med Chem Lett (2019) 10 : 1524 -1529
PubMed 31749905 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 5.45 5.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4483695 1 5.52
CHEMBL4448916 1 5.51
CHEMBL4469631 1 5.33
CHEMBL4439536 1 -
CHEMBL4540185 1 -
CHEMBL4552727 1 -
CHEMBL4444464 1 -
CHEMBL4571655 1 -
CHEMBL4457730 1 -
CHEMBL4441045 1 -
CHEMBL4541056 1 -
CHEMBL4561577 1 -
CHEMBL4556614 1 -
CHEMBL4451769 1 -
CHEMBL4456070 1 -
CHEMBL4586676 1 -
CHEMBL4532502 1 -
CHEMBL4575553 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4483695 IC50 = 3040.0 nM 5.52
CHEMBL4448916 IC50 = 3110.0 nM 5.51
CHEMBL4469631 IC50 = 4690.0 nM 5.33
CHEMBL4439536 IC50 > 25000.0 nM -
CHEMBL4540185 IC50 > 25000.0 nM -
CHEMBL4552727 IC50 > 25000.0 nM -
CHEMBL4444464 IC50 > 25000.0 nM -
CHEMBL4571655 IC50 > 25000.0 nM -
CHEMBL4457730 IC50 > 25000.0 nM -
CHEMBL4441045 IC50 > 25000.0 nM -
CHEMBL4541056 IC50 > 25000.0 nM -
CHEMBL4561577 IC50 > 25000.0 nM -
CHEMBL4556614 IC50 > 25000.0 nM -
CHEMBL4451769 IC50 > 25000.0 nM -
CHEMBL4456070 IC50 > 25000.0 nM -
CHEMBL4586676 IC50 > 25000.0 nM -
CHEMBL4532502 IC50 > 25000.0 nM -
CHEMBL4575553 IC50 > 25000.0 nM -