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Assay Detail

CHEMBL4411096

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of BODIPY TR-ADP from bovine GRK1 (1 to 535 residues) preincubated for 10 mins followed by compound addition and measured after 10 to 15 mins by fluorescence polarization displacement assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Bos taurus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Rhodopsin kinase GRK1 (CHEMBL3879860)
Type SINGLE PROTEIN
Organism Bos taurus

Publication

Structure-Based Design of Selective, Covalent G Protein-Coupled Receptor Kinase 5 Inhibitors.
Rowlands RA, Cato MC, Waldschmidt HV, Bouley RA, Chen Q, Avramova L, Larsen SD, Tesmer JJG, White AD.
ACS Med Chem Lett (2019) 10 : 1628 -1634
PubMed 31857838 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 5.89 6.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL463931 GSK-2163632A 1 6.89
CHEMBL517171 GSK-1713088A 1 4.89
CHEMBL476259 GSK-1326255A 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL463931 GSK-2163632A IC50 = 130.0 nM 6.89
CHEMBL517171 GSK-1713088A IC50 = 13000.0 nM 4.89
CHEMBL476259 GSK-1326255A IC50 > 100000.0 nM -