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Assay Detail

CHEMBL4418432

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of CYP8B1 in human liver microsomes using D7-7alpha-hydroxy-4-cholesten-3-one as substrate preincubated for 20 mins followed by substrate addition in presence of NADPH by UPLC-ESI-MS/MS analysis
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Tissue Liver
Subcellular Microsomes
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Cyp8b1 and uses thereof in therapeutic and diagnostic methods
(2014)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 5.63 6.51

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL808 ECONAZOLE 4.0 1 6.51
CHEMBL104 CLOTRIMAZOLE 4.0 1 6.10
CHEMBL91 MICONAZOLE 4.0 1 5.82
CHEMBL157101 KETOCONAZOLE 4.0 1 5.66
CHEMBL4442751 1 5.58
CHEMBL1397 POSACONAZOLE 4.0 1 5.42
CHEMBL64391 ITRACONAZOLE 4.0 1 5.33
CHEMBL4468145 1 5.12
CHEMBL3989843 TRANYLCYPROMINE 4.0 1 5.11

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL808 ECONAZOLE IC50 = 310.0 nM 6.51
CHEMBL104 CLOTRIMAZOLE IC50 = 800.0 nM 6.10
CHEMBL91 MICONAZOLE IC50 = 1500.0 nM 5.82
CHEMBL157101 KETOCONAZOLE IC50 = 2200.0 nM 5.66
CHEMBL4442751 IC50 = 2600.0 nM 5.58
CHEMBL1397 POSACONAZOLE IC50 = 3800.0 nM 5.42
CHEMBL64391 ITRACONAZOLE IC50 = 4700.0 nM 5.33
CHEMBL4468145 IC50 = 7600.0 nM 5.12
CHEMBL3989843 TRANYLCYPROMINE IC50 = 7800.0 nM 5.11