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Assay Detail

CHEMBL4422548

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of GST-fused human Eya2 ED (253 to 538 residues) expressed in Escherichia coli pre-incubated for 10 mins before phosphorylated H2AX peptide substrate addition and further incubated for 40 mins by malachite green-based secondary phosphatase assay
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Eyes absent homolog 2 (CHEMBL1293275)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Inhibitors of eya2
(2012)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 4.76 4.78

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4459891 1 4.78
CHEMBL1975051 1 4.78
CHEMBL3195222 1 4.78
CHEMBL4445513 1 4.78
CHEMBL3189517 1 4.78
CHEMBL3190554 1 4.78
CHEMBL4446792 1 4.78
CHEMBL4476815 1 4.78
CHEMBL4446652 1 4.78
CHEMBL3191598 1 4.78
CHEMBL4530754 1 4.78
CHEMBL4458563 1 4.74
CHEMBL4532983 1 4.50

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4459891 IC50 = 16800.0 nM 4.78
CHEMBL1975051 IC50 = 16800.0 nM 4.78
CHEMBL3195222 IC50 = 16800.0 nM 4.78
CHEMBL4445513 IC50 = 16800.0 nM 4.78
CHEMBL3189517 IC50 = 16800.0 nM 4.78
CHEMBL3190554 IC50 = 16800.0 nM 4.78
CHEMBL4446792 IC50 = 16800.0 nM 4.78
CHEMBL4476815 IC50 = 16800.0 nM 4.78
CHEMBL4446652 IC50 = 16800.0 nM 4.78
CHEMBL3191598 IC50 = 16800.0 nM 4.78
CHEMBL4530754 IC50 = 16800.0 nM 4.78
CHEMBL4458563 IC50 = 18250.0 nM 4.74
CHEMBL4532983 IC50 = 31980.0 nM 4.50