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Assay Detail

CHEMBL4425965

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]DHA from inactive/G protein-uncoupled human beta2-AR expressed in CHO cell membranes assessed as intrinsic Kd by liquid scintillation counting
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-2 adrenergic receptor (CHEMBL210)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Uncoupling the Structure-Activity Relationships of β2 Adrenergic Receptor Ligands from Membrane Binding.
Dickson CJ, Hornak V, Velez-Vega C, McKay DJ, Reilly J, Sandham DA, Shaw D, Fairhurst RA, Charlton SJ, Sykes DA, Pearlstein RA, Duca JS.
J Med Chem (2016) 59 : 5780 -5789
PubMed 27239696 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 11 6.38 9.15

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL378501 CYANOPINDOLOL 1 9.15
CHEMBL420746 CGP-12177 1 7.72
CHEMBL521606 NADOLOL 4.0 1 7.16
CHEMBL27 PROPRANOLOL 4.0 1 6.82
CHEMBL1233766 1 6.20
CHEMBL434 ISOPROTERENOL 4.0 1 6.13
CHEMBL1263 SALMETEROL 4.0 1 5.96
CHEMBL24 ATENOLOL 4.0 1 5.92
CHEMBL714 ALBUTEROL 4.0 1 5.85
CHEMBL13 METOPROLOL 4.0 1 4.81
CHEMBL645 BISOPROLOL 4.0 1 4.48

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL378501 CYANOPINDOLOL Kd = 0.7 nM 9.15
CHEMBL420746 CGP-12177 Kd = 19.0 nM 7.72
CHEMBL521606 NADOLOL Kd = 70.0 nM 7.16
CHEMBL27 PROPRANOLOL Kd = 150.0 nM 6.82
CHEMBL1233766 Kd = 630.0 nM 6.20
CHEMBL434 ISOPROTERENOL Kd = 740.0 nM 6.13
CHEMBL1263 SALMETEROL Kd = 1100.0 nM 5.96
CHEMBL24 ATENOLOL Kd = 1200.0 nM 5.92
CHEMBL714 ALBUTEROL Kd = 1400.0 nM 5.85
CHEMBL13 METOPROLOL Kd = 15500.0 nM 4.81
CHEMBL645 BISOPROLOL Kd = 33000.0 nM 4.48