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Assay Detail

CHEMBL4480512

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of ovine COX1 using arachidonic acid as substrate preincubated for 10 mins followed by substrate addition and measured after 2 mins by ELISA
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Ovis aries
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Prostaglandin G/H synthase 1 (CHEMBL2949)
Type SINGLE PROTEIN
Organism Ovis aries

Publication

Rationally synthesized coumarin based pyrazolines ameliorate carrageenan induced inflammation through COX-2/pro-inflammatory cytokine inhibition.
Chandel P, Kumar A, Singla N, Kumar A, Singh G, Gill RK.
Medchemcomm (2019) 10 : 421 -430
PubMed 30996860 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 4.71 4.79

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4519601 1 4.79
CHEMBL1258748 1 4.70
CHEMBL1258747 1 4.65
CHEMBL4483384 1 -
CHEMBL4531675 1 -
CHEMBL1258869 1 -
CHEMBL4589730 1 -
CHEMBL416146 ETORICOXIB 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4519601 IC50 = 16200.0 nM 4.79
CHEMBL1258748 IC50 = 20000.0 nM 4.70
CHEMBL1258747 IC50 = 22400.0 nM 4.65
CHEMBL4483384 IC50 > 30000.0 nM -
CHEMBL4531675 IC50 - -
CHEMBL1258869 IC50 - -
CHEMBL4589730 IC50 > 30000.0 nM -
CHEMBL416146 ETORICOXIB IC50 > 30000.0 nM -