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Assay Detail

CHEMBL4605648

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Inhibition of human recombinant CYP3A4 expressed in insect cell microsomes in presence of NADPH by fluorescence assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Subcellular Microsomes
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cytochrome P450 3A4 (CHEMBL340)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Accelerated Discovery of Novel Ponatinib Analogs with Improved Properties for the Treatment of Parkinson's Disease.
Kaiser TM, Dentmon ZW, Dalloul CE, Sharma SK, Liotta DC.
ACS Med Chem Lett (2020) 11 : 491 -496
PubMed 32292555 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 5.20 6.24

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL255863 NILOTINIB 4.0 1 6.24
CHEMBL4633367 1 5.34
CHEMBL4640297 1 5.25
CHEMBL4638981 1 5.14
CHEMBL1171837 PONATINIB 4.0 1 4.94
CHEMBL4632471 1 4.93
CHEMBL4637502 1 4.87
CHEMBL4642144 1 4.87

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL255863 NILOTINIB IC50 = 580.0 nM 6.24
CHEMBL4633367 IC50 = 4610.0 nM 5.34
CHEMBL4640297 IC50 = 5580.0 nM 5.25
CHEMBL4638981 IC50 = 7280.0 nM 5.14
CHEMBL1171837 PONATINIB IC50 = 11400.0 nM 4.94
CHEMBL4632471 IC50 = 11700.0 nM 4.93
CHEMBL4637502 IC50 = 13500.0 nM 4.87
CHEMBL4642144 IC50 = 13400.0 nM 4.87