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Assay Detail

CHEMBL4611051

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at TLR7 in mouse RAW264.7 cells assessed as immunostimulatory activity by measuring increase in TNFalpha secretion incubated for 18 hrs by ELISA
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type RAW264.7
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Toll-like receptor 7 (CHEMBL6085)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Synthesis and immunostimulatory activity of sugar-conjugated TLR7 ligands.
Baba A, Wakao M, Shinchi H, Chan M, Hayashi T, Yao S, Cottam HB, Carson DA, Suda Y.
Bioorg Med Chem Lett (2020) 30 : 126840 -126840
PubMed 31864800 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 14 6.83 7.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4635169 1 7.40
CHEMBL4635551 1 7.24
CHEMBL4641702 1 7.10
CHEMBL4636482 1 6.97
CHEMBL4639831 1 6.89
CHEMBL4636020 1 6.88
CHEMBL4645661 1 6.87
CHEMBL4632609 1 6.82
CHEMBL4641619 1 6.64
CHEMBL4640564 1 6.63
CHEMBL4637769 1 6.60
CHEMBL4647035 1 6.60
CHEMBL4647802 1 6.54
CHEMBL4639099 1 6.50

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4635169 EC50 = 40.0 nM 7.40
CHEMBL4635551 EC50 = 57.0 nM 7.24
CHEMBL4641702 EC50 = 80.0 nM 7.10
CHEMBL4636482 EC50 = 107.0 nM 6.97
CHEMBL4639831 EC50 = 128.0 nM 6.89
CHEMBL4636020 EC50 = 132.0 nM 6.88
CHEMBL4645661 EC50 = 136.0 nM 6.87
CHEMBL4632609 EC50 = 153.0 nM 6.82
CHEMBL4641619 EC50 = 230.0 nM 6.64
CHEMBL4640564 EC50 = 234.0 nM 6.63
CHEMBL4637769 EC50 = 252.0 nM 6.60
CHEMBL4647035 EC50 = 252.0 nM 6.60
CHEMBL4647802 EC50 = 287.0 nM 6.54
CHEMBL4639099 EC50 = 316.0 nM 6.50