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Assay Detail

CHEMBL4616933

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Induction of morphological changes in human T47D cells assessed as number of nuclei incubated for 48 hrs by Hoechst 33342, SYTO14, Phalloidin-594, Wheat germ agglutinin-594, Concavalin-488, MitoTracker DeepRed staining based wide field fluorescent microscopic method
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type T47D
Confidence 1 — Organism
Curated By Autocuration

Target

T47D (CHEMBL614361)
Type CELL-LINE
Organism Homo sapiens

Publication

High content phenotypic screening identifies serotonin receptor modulators with selective activity upon breast cancer cell cycle and cytokine signaling pathways.
Warchal SJ, Dawson JC, Shepherd E, Munro AF, Hughes RE, Makda A, Carragher NO.
Bioorg Med Chem (2020) 28 : 115209 -115209
PubMed 31757681 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 2 5.19 5.27

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL570 TRIFLUPROMAZINE 4.0 1 5.27
CHEMBL668 PROTRIPTYLINE 4.0 1 5.11

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL570 TRIFLUPROMAZINE EC50 = 5400.0 nM 5.27
CHEMBL668 PROTRIPTYLINE EC50 = 7700.0 nM 5.11