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Assay Detail

CHEMBL4674512

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of GLK (unknown origin) transfected in human 293T cells co-transfected with GFP-fused PKCtheta assessed as reduction in PKCtheta phosphorylation by ELISA
18
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293T
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Investigating small molecules to inhibit germinal center kinase-like kinase (GLK/MAP4K3) upstream of PKCθ phosphorylation: Potential therapy to modulate T cell dependent immunity.
May-Dracka TL,Arduini R,Bertolotti-Ciarlet A,Bhisetti G,Brickelmaier M,Cahir-McFarland E,Enyedy I,Fontenot JD,Hesson T,Little K,Lyssikatos J,Marcotte D,McKee T,Murugan P,Patterson T,Peng H,Rushe M,Silvian L,Spilker K,Wu P,Xin Z,Burkly LC
Bioorg Med Chem Lett (2018) 28 : 1964 -1971
PubMed 29636220 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 6.25 7.11

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4793665 1 7.11
CHEMBL4761177 1 6.59
CHEMBL4749141 1 6.56
CHEMBL4753188 1 6.31
CHEMBL4785602 1 6.19
CHEMBL4568440 2 6.00
CHEMBL4762312 1 5.91
CHEMBL4782885 1 5.85
CHEMBL601719 CRIZOTINIB 4.0 1 5.77
CHEMBL518323 1 -
CHEMBL4744805 1 -
CHEMBL4779709 1 -
CHEMBL4787817 1 -
CHEMBL4741011 1 -
CHEMBL4794231 1 -
CHEMBL4749627 1 -
CHEMBL1825141 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4793665 IC50 = 78.0 nM 7.11
CHEMBL4761177 IC50 = 258.0 nM 6.59
CHEMBL4749141 IC50 = 278.0 nM 6.56
CHEMBL4753188 IC50 = 490.0 nM 6.31
CHEMBL4785602 IC50 = 645.0 nM 6.19
CHEMBL4568440 IC50 = 1000.0 nM 6.00
CHEMBL4762312 IC50 = 1220.0 nM 5.91
CHEMBL4782885 IC50 = 1400.0 nM 5.85
CHEMBL601719 CRIZOTINIB IC50 = 1700.0 nM 5.77
CHEMBL1825141 IC50 - -
CHEMBL4568440 IC50 - -
CHEMBL4749627 IC50 - -
CHEMBL4794231 IC50 - -
CHEMBL4787817 IC50 - -
CHEMBL4779709 IC50 - -
CHEMBL4744805 IC50 - -
CHEMBL4741011 IC50 - -
CHEMBL518323 IC50 - -