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Assay Detail

CHEMBL4678924

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant full length human N-terminal GST-fused CDK9 (1 to 372 residues)/His-tagged CyclinT1 (1 to 726 residues) expressed in baculovirus infected insect cells using FITC-X-GSRTPMY-NH2 as substrate preincubated for 10 mins followed by MgCl2 addition and measured after 90 mins in presence of 5 mM ATP by mobility shift assay
37
Total Activities
35
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

CDK9/cyclin T1 (CHEMBL2111389)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Discovery of AZD4573, a Potent and Selective Inhibitor of CDK9 That Enables Short Duration of Target Engagement for the Treatment of Hematological Malignancies.
Barlaam B,Casella R,Cidado J,Cook C,De Savi C,Dishington A,Donald CS,Drew L,Ferguson AD,Ferguson D,Glossop S,Grebe T,Gu C,Hande S,Hawkins J,Hird AW,Holmes J,Horstick J,Jiang Y,Lamb ML,McGuire TM,Moore JE,O'Connell N,Pike A,Pike KG,Proia T,Roberts B,San Martin M,Sarkar U,Shao W,Stead D,Sumner N,Thakur K,Vasbinder MM,Varnes JG,Wang J,Wang L,Wu D,Wu L,Yang B,Yao T
J Med Chem (2020) 63 : 15564 -15590
PubMed 33306391 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 37 7.01 8.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4784117 1 8.52
CHEMBL4754081 1 8.40
CHEMBL4785242 1 8.10
CHEMBL4749175 1 7.96
CHEMBL4759074 2 7.77
CHEMBL4747227 1 7.70
CHEMBL4753076 1 7.66
CHEMBL4786995 1 7.64
CHEMBL4740254 1 7.60
CHEMBL4753256 2 7.58
CHEMBL3673434 1 7.54
CHEMBL4741063 1 7.54
CHEMBL4754399 1 7.41
CHEMBL4751230 1 7.29
CHEMBL4750372 1 7.22
CHEMBL4784363 1 7.21
CHEMBL4759053 1 7.21
CHEMBL4777517 1 7.07
CHEMBL4796500 1 6.96
CHEMBL4792521 1 6.86
CHEMBL4788016 1 6.75
CHEMBL4764361 1 6.66
CHEMBL4780912 1 6.64
CHEMBL4758821 1 6.57
CHEMBL4798156 1 6.54
CHEMBL4756050 1 6.51
CHEMBL4750648 1 6.46
CHEMBL4760601 1 6.42
CHEMBL4789009 1 6.18
CHEMBL4765079 1 6.12

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4784117 IC50 = 3.0 nM 8.52
CHEMBL4754081 IC50 = 4.0 nM 8.40
CHEMBL4785242 IC50 = 8.0 nM 8.10
CHEMBL4749175 IC50 = 11.0 nM 7.96
CHEMBL4759074 IC50 = 17.0 nM 7.77
CHEMBL4747227 IC50 = 20.0 nM 7.70
CHEMBL4753076 IC50 = 22.0 nM 7.66
CHEMBL4786995 IC50 = 23.0 nM 7.64
CHEMBL4740254 IC50 = 25.0 nM 7.60
CHEMBL4753256 IC50 = 26.0 nM 7.58
CHEMBL4753256 IC50 = 27.0 nM 7.57
CHEMBL3673434 IC50 = 29.0 nM 7.54
CHEMBL4741063 IC50 = 29.0 nM 7.54
CHEMBL4754399 IC50 = 39.0 nM 7.41
CHEMBL4751230 IC50 = 51.0 nM 7.29
CHEMBL4750372 IC50 = 60.0 nM 7.22
CHEMBL4784363 IC50 = 62.0 nM 7.21
CHEMBL4759053 IC50 = 61.0 nM 7.21
CHEMBL4777517 IC50 = 85.0 nM 7.07
CHEMBL4796500 IC50 = 110.0 nM 6.96
CHEMBL4792521 IC50 = 139.0 nM 6.86
CHEMBL4788016 IC50 = 180.0 nM 6.75
CHEMBL4764361 IC50 = 220.0 nM 6.66
CHEMBL4780912 IC50 = 230.0 nM 6.64
CHEMBL4758821 IC50 = 270.0 nM 6.57
CHEMBL4798156 IC50 = 290.0 nM 6.54
CHEMBL4756050 IC50 = 310.0 nM 6.51
CHEMBL4750648 IC50 = 350.0 nM 6.46
CHEMBL4760601 IC50 = 380.0 nM 6.42
CHEMBL4789009 IC50 = 660.0 nM 6.18
CHEMBL4765079 IC50 = 750.0 nM 6.12
CHEMBL4787309 IC50 = 1000.0 nM 6.00
CHEMBL4764938 IC50 = 4400.0 nM 5.36
CHEMBL4799201 IC50 = 5300.0 nM 5.28
CHEMBL4759074 IC50 = 6800.0 nM 5.17
CHEMBL4752748 IC50 < 3.0 nM -
CHEMBL4462530 ZEMIRCICLIB IC50 < 4.0 nM -