Assay Detail
Binding
CHEMBL4680701
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of PRMT5 in human Z138 cells assessed as reduction in symmetrical dimethylation of arginine containing substrate using SmD3 as substrate incubated for 4 days by In-cell western assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Z138 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Protein arginine N-methyltransferase 5 (CHEMBL1795116) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Structure and Property Guided Design in the Identification of PRMT5 Tool Compound EPZ015666.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 12 | 7.33 | 8.52 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4542380 | — | — | 1 | 8.52 |
| CHEMBL4060831 | — | — | 1 | 8.05 |
| CHEMBL3938760 | — | — | 1 | 7.89 |
| CHEMBL3958947 | — | — | 1 | 7.80 |
| CHEMBL3963350 | — | — | 1 | 7.50 |
| CHEMBL3919677 | — | — | 1 | 7.44 |
| CHEMBL3927051 | — | — | 1 | 7.43 |
| CHEMBL3913586 | — | — | 1 | 6.75 |
| CHEMBL3962874 | — | — | 1 | 6.49 |
| CHEMBL3914119 | — | — | 1 | 6.39 |
| CHEMBL3977219 | — | — | 1 | 6.36 |
| CHEMBL3890510 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4542380 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL4060831 | — | IC50 | = | 9.0 | nM | 8.05 |
| CHEMBL3938760 | — | IC50 | = | 13.0 | nM | 7.89 |
| CHEMBL3958947 | — | IC50 | = | 16.0 | nM | 7.80 |
| CHEMBL3963350 | — | IC50 | = | 32.0 | nM | 7.50 |
| CHEMBL3919677 | — | IC50 | = | 36.0 | nM | 7.44 |
| CHEMBL3927051 | — | IC50 | = | 37.0 | nM | 7.43 |
| CHEMBL3913586 | — | IC50 | = | 180.0 | nM | 6.75 |
| CHEMBL3962874 | — | IC50 | = | 327.0 | nM | 6.49 |
| CHEMBL3914119 | — | IC50 | = | 406.0 | nM | 6.39 |
| CHEMBL3977219 | — | IC50 | = | 435.0 | nM | 6.36 |
| CHEMBL3890510 | — | IC50 | - | — | - |