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Assay Detail

CHEMBL4728927

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of AlexaFluor-labeled tracer 236 binding to recombinant human His-tagged CSF1R (538 to 910 residues) preincubated for 20 mins followed by Eu-labeled anti-His antibody addition and measured after 90 mins by FRET based LanthaScreen assay
33
Total Activities
33
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Macrophage colony-stimulating factor 1 receptor (CHEMBL1844)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of IACS-9439, a Potent, Exquisitely Selective, and Orally Bioavailable Inhibitor of CSF1R.
Czako B,Marszalek JR,Burke JP,Mandal P,Leonard PG,Cross JB,Mseeh F,Jiang Y,Chang EQ,Suzuki E,Kovacs JJ,Feng N,Gera S,Harris AL,Liu Z,Mullinax RA,Pang J,Parker CA,Spencer ND,Yu SS,Wu Q,Tremblay MR,Mikule K,Wilcoxen K,Heffernan TP,Draetta GF,Jones P
J Med Chem (2020) 63 : 9888 -9911
PubMed 32787110 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 33 7.84 9.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4758092 1 9.10
CHEMBL4751287 1 9.00
CHEMBL4763875 1 9.00
CHEMBL4798876 1 8.96
CHEMBL4795462 1 8.96
CHEMBL4798601 1 8.92
CHEMBL4740157 1 8.92
CHEMBL4759047 1 8.85
CHEMBL4748740 1 8.77
CHEMBL4741283 1 8.70
CHEMBL4779878 1 8.70
CHEMBL4756510 1 8.62
CHEMBL4779197 1 8.59
CHEMBL4754431 1 8.40
CHEMBL4746711 1 8.40
CHEMBL4788945 1 8.40
CHEMBL4763289 1 8.24
CHEMBL4762843 1 8.15
CHEMBL4787609 1 8.05
CHEMBL4790139 1 7.96
CHEMBL4798920 1 7.77
CHEMBL4788832 1 7.68
CHEMBL4764974 1 7.52
CHEMBL4743008 1 7.32
CHEMBL4777344 1 7.28
CHEMBL4751359 1 7.12
CHEMBL4781950 1 6.52
CHEMBL4797083 1 6.35
CHEMBL4783368 1 6.20
CHEMBL4742978 1 6.18

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4758092 IC50 = 0.8 nM 9.10
CHEMBL4751287 IC50 = 1.0 nM 9.00
CHEMBL4763875 IC50 = 1.0 nM 9.00
CHEMBL4798876 IC50 = 1.1 nM 8.96
CHEMBL4795462 IC50 = 1.1 nM 8.96
CHEMBL4798601 IC50 = 1.2 nM 8.92
CHEMBL4740157 IC50 = 1.2 nM 8.92
CHEMBL4759047 IC50 = 1.4 nM 8.85
CHEMBL4748740 IC50 = 1.7 nM 8.77
CHEMBL4741283 IC50 = 2.0 nM 8.70
CHEMBL4779878 IC50 = 2.0 nM 8.70
CHEMBL4756510 IC50 = 2.4 nM 8.62
CHEMBL4779197 IC50 = 2.6 nM 8.59
CHEMBL4788945 IC50 = 4.0 nM 8.40
CHEMBL4746711 IC50 = 4.0 nM 8.40
CHEMBL4754431 IC50 = 4.0 nM 8.40
CHEMBL4763289 IC50 = 5.7 nM 8.24
CHEMBL4762843 IC50 = 7.0 nM 8.15
CHEMBL4787609 IC50 = 9.0 nM 8.05
CHEMBL4790139 IC50 = 11.0 nM 7.96
CHEMBL4798920 IC50 = 17.0 nM 7.77
CHEMBL4788832 IC50 = 21.0 nM 7.68
CHEMBL4764974 IC50 = 30.0 nM 7.52
CHEMBL4743008 IC50 = 48.0 nM 7.32
CHEMBL4777344 IC50 = 52.0 nM 7.28
CHEMBL4751359 IC50 = 76.0 nM 7.12
CHEMBL4781950 IC50 = 303.0 nM 6.52
CHEMBL4797083 IC50 = 450.0 nM 6.35
CHEMBL4783368 IC50 = 626.0 nM 6.20
CHEMBL4742978 IC50 = 655.0 nM 6.18
CHEMBL4745859 IC50 = 781.0 nM 6.11
CHEMBL4742128 IC50 = 4413.0 nM 5.36
CHEMBL4791937 IC50 = 27712.0 nM 4.56