Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL4730248

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of fluormone from GST-tagged ERbeta receptor LBD (unknown origin) measured after 60 mins by TR-FRET competitive binding assay
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Estrogen receptor beta (CHEMBL242)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and evaluation of 17α-triazolyl and 9α-cyano derivatives of estradiol.
Wetzel EA,Hanson AM,Troutfetter CL,Burkett DJ,Sem DS,Donaldson WA
Bioorg Med Chem (2020) 28 : 115670 -115670
PubMed 32912438 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 14 7.85 9.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL135 ESTRADIOL 4.0 1 9.68
CHEMBL4782490 1 9.57
CHEMBL691 ETHINYL ESTRADIOL 4.0 1 9.43
CHEMBL4761096 1 9.19
CHEMBL1405 ESTRONE 4.0 1 9.03
CHEMBL4785948 1 8.22
CHEMBL4752614 1 7.92
CHEMBL4778702 1 7.82
CHEMBL4745013 1 7.30
CHEMBL4751119 1 7.29
CHEMBL4744639 1 7.11
CHEMBL4741481 1 6.44
CHEMBL4754151 1 5.55
CHEMBL4747395 1 5.36

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL135 ESTRADIOL EC50 = 0.21 nM 9.68
CHEMBL4782490 EC50 = 0.27 nM 9.57
CHEMBL691 ETHINYL ESTRADIOL EC50 = 0.37 nM 9.43
CHEMBL4761096 EC50 = 0.65 nM 9.19
CHEMBL1405 ESTRONE EC50 = 0.94 nM 9.03
CHEMBL4785948 EC50 = 6.0 nM 8.22
CHEMBL4752614 EC50 = 12.0 nM 7.92
CHEMBL4778702 EC50 = 15.0 nM 7.82
CHEMBL4745013 EC50 = 50.0 nM 7.30
CHEMBL4751119 EC50 = 51.0 nM 7.29
CHEMBL4744639 EC50 = 78.0 nM 7.11
CHEMBL4741481 EC50 = 360.0 nM 6.44
CHEMBL4754151 EC50 = 2800.0 nM 5.55
CHEMBL4747395 EC50 = 4400.0 nM 5.36