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Assay Detail

CHEMBL4730249

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at full length human ERalpha receptor assessed as transcriptional activity incubated for 22 to 24 hrs by cell based luciferase reporter gene assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Estrogen receptor (CHEMBL206)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and evaluation of 17α-triazolyl and 9α-cyano derivatives of estradiol.
Wetzel EA,Hanson AM,Troutfetter CL,Burkett DJ,Sem DS,Donaldson WA
Bioorg Med Chem (2020) 28 : 115670 -115670
PubMed 32912438 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 11 8.30 9.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL691 ETHINYL ESTRADIOL 4.0 1 9.52
CHEMBL135 ESTRADIOL 4.0 1 9.51
CHEMBL4782490 1 9.42
CHEMBL4761096 1 9.06
CHEMBL1405 ESTRONE 4.0 1 8.62
CHEMBL4785948 1 8.30
CHEMBL4778702 1 8.15
CHEMBL4751119 1 7.66
CHEMBL4752614 1 7.17
CHEMBL4744639 1 7.06
CHEMBL4745013 1 6.83

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL691 ETHINYL ESTRADIOL EC50 = 0.3 nM 9.52
CHEMBL135 ESTRADIOL EC50 = 0.31 nM 9.51
CHEMBL4782490 EC50 = 0.38 nM 9.42
CHEMBL4761096 EC50 = 0.87 nM 9.06
CHEMBL1405 ESTRONE EC50 = 2.4 nM 8.62
CHEMBL4785948 EC50 = 5.0 nM 8.30
CHEMBL4778702 EC50 = 7.1 nM 8.15
CHEMBL4751119 EC50 = 22.0 nM 7.66
CHEMBL4752614 EC50 = 68.0 nM 7.17
CHEMBL4744639 EC50 = 88.0 nM 7.06
CHEMBL4745013 EC50 = 149.0 nM 6.83