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Assay Detail

CHEMBL4808435

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of PRMT5 (unknown origin) assessed as inhibition of symmetric dimethylation of arginine incubated for 3 days by fluorescence based cellular target engagement assay
33
Total Activities
33
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Protein arginine N-methyltransferase 5 (CHEMBL1795116)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The Discovery of Two Novel Classes of 5,5-Bicyclic Nucleoside-Derived PRMT5 Inhibitors for the Treatment of Cancer.
Quiroz RV, Reutershan MH, Schneider SE, Sloman D, Lacey BM, Swalm BM, Yeung CS, Gibeau C, Spellman DS, Rankic DA, Chen D, Witter D, Linn D, Munsell E, Feng G, Xu H, Hughes JME, Lim J, Saurí J, Geddes K, Wan M, Mansueto MS, Follmer NE, Fier PS, Siliphaivanh P, Daublain P, Palte RL, Hayes RP, Lee S, Kawamura S, Silverman S, Sanyal S, Henderson TJ, Ye Y, Gao Y, Nicholson B, Machacek MR.
J Med Chem (2021) 64 : 3911 -3939
PubMed 33755451 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 33 8.51 9.13

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4854745 1 9.13
CHEMBL4861625 1 9.08
CHEMBL4867460 1 9.06
CHEMBL4860414 1 9.05
CHEMBL4852843 1 9.05
CHEMBL4873949 1 9.01
CHEMBL4868904 1 8.96
CHEMBL4853705 1 8.92
CHEMBL4867267 1 8.92
CHEMBL4846860 1 8.92
CHEMBL4851358 1 8.89
CHEMBL4847518 1 8.82
CHEMBL4878171 1 8.77
CHEMBL4860893 1 8.64
CHEMBL4874354 1 8.60
CHEMBL4850190 1 8.55
CHEMBL4846178 1 8.54
CHEMBL4853843 1 8.49
CHEMBL4849342 1 8.49
CHEMBL4869259 1 8.40
CHEMBL4873952 1 8.34
CHEMBL4868831 1 8.33
CHEMBL4849067 1 8.28
CHEMBL4854567 1 8.28
CHEMBL4862596 1 8.00
CHEMBL4853251 1 8.00
CHEMBL4862485 1 7.96
CHEMBL4848739 1 7.85
CHEMBL4872775 1 7.68
CHEMBL4864483 1 7.50

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4854745 EC50 = 0.74 nM 9.13
CHEMBL4861625 EC50 = 0.84 nM 9.08
CHEMBL4867460 EC50 = 0.87 nM 9.06
CHEMBL4860414 EC50 = 0.9 nM 9.05
CHEMBL4852843 EC50 = 0.89 nM 9.05
CHEMBL4873949 EC50 = 0.97 nM 9.01
CHEMBL4868904 EC50 = 1.1 nM 8.96
CHEMBL4853705 EC50 = 1.2 nM 8.92
CHEMBL4867267 EC50 = 1.2 nM 8.92
CHEMBL4846860 EC50 = 1.2 nM 8.92
CHEMBL4851358 EC50 = 1.3 nM 8.89
CHEMBL4847518 EC50 = 1.5 nM 8.82
CHEMBL4878171 EC50 = 1.7 nM 8.77
CHEMBL4860893 EC50 = 2.3 nM 8.64
CHEMBL4874354 EC50 = 2.5 nM 8.60
CHEMBL4850190 EC50 = 2.8 nM 8.55
CHEMBL4846178 EC50 = 2.9 nM 8.54
CHEMBL4853843 EC50 = 3.2 nM 8.49
CHEMBL4849342 EC50 = 3.2 nM 8.49
CHEMBL4869259 EC50 = 4.0 nM 8.40
CHEMBL4873952 EC50 = 4.6 nM 8.34
CHEMBL4868831 EC50 = 4.7 nM 8.33
CHEMBL4849067 EC50 = 5.3 nM 8.28
CHEMBL4854567 EC50 = 5.3 nM 8.28
CHEMBL4862596 EC50 = 9.9 nM 8.00
CHEMBL4853251 EC50 = 9.9 nM 8.00
CHEMBL4862485 EC50 = 11.0 nM 7.96
CHEMBL4848739 EC50 = 14.0 nM 7.85
CHEMBL4872775 EC50 = 21.0 nM 7.68
CHEMBL4864483 EC50 = 32.0 nM 7.50
CHEMBL4868773 EC50 = 38.0 nM 7.42
CHEMBL4862310 EC50 > 10.0 nM -
CHEMBL4874774 EC50 < 0.5 nM -