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Assay Detail

CHEMBL4810079

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant NNMT using nicotinamide and SAM as substrate assessed as reduction in 1-methyl-nicotinamide formation incubated for 22 to 24 hrs by RapidFire Mass spectroscopy
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nicotinamide N-methyltransferase (CHEMBL2346486)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Macrocyclic Peptides as a Novel Class of NNMT Inhibitors: A SAR Study Aimed at Inhibitory Activity in the Cell.
Hayashi K, Uehara S, Yamamoto S, Cary DR, Nishikawa J, Ueda T, Ozasa H, Mihara K, Yoshimura N, Kawai T, Ono T, Yamamoto S, Fumoto M, Mikamiyama H.
ACS Med Chem Lett (2021) 12 : 1093 -1101
PubMed 34267879 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 27 7.56 9.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4867273 1 9.82
CHEMBL4859806 1 9.40
CHEMBL4847740 1 9.33
CHEMBL4857394 1 9.03
CHEMBL4878738 1 8.96
CHEMBL4872872 1 8.72
CHEMBL4856854 1 8.24
CHEMBL4848464 1 7.85
CHEMBL4853328 1 7.85
CHEMBL4868588 1 7.55
CHEMBL4859039 1 7.44
CHEMBL4849636 1 7.38
CHEMBL4858283 1 7.35
CHEMBL4868364 1 7.28
CHEMBL4863816 1 7.21
CHEMBL4875414 1 6.97
CHEMBL4870987 1 6.90
CHEMBL4860372 1 6.85
CHEMBL4873940 1 6.84
CHEMBL4878905 1 6.67
CHEMBL4851654 1 6.66
CHEMBL4856886 1 6.19
CHEMBL4868547 1 5.57
CHEMBL4869822 1 5.38
CHEMBL4856096 1 -
CHEMBL4875995 1 -
CHEMBL4859787 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4867273 IC50 = 0.15 nM 9.82
CHEMBL4859806 IC50 = 0.4 nM 9.40
CHEMBL4847740 IC50 = 0.47 nM 9.33
CHEMBL4857394 IC50 = 0.93 nM 9.03
CHEMBL4878738 IC50 = 1.1 nM 8.96
CHEMBL4872872 IC50 = 1.9 nM 8.72
CHEMBL4856854 IC50 = 5.8 nM 8.24
CHEMBL4848464 IC50 = 14.0 nM 7.85
CHEMBL4853328 IC50 = 14.0 nM 7.85
CHEMBL4868588 IC50 = 28.0 nM 7.55
CHEMBL4859039 IC50 = 36.0 nM 7.44
CHEMBL4849636 IC50 = 42.0 nM 7.38
CHEMBL4858283 IC50 = 45.0 nM 7.35
CHEMBL4868364 IC50 = 52.0 nM 7.28
CHEMBL4863816 IC50 = 61.0 nM 7.21
CHEMBL4875414 IC50 = 108.0 nM 6.97
CHEMBL4870987 IC50 = 125.0 nM 6.90
CHEMBL4860372 IC50 = 141.0 nM 6.85
CHEMBL4873940 IC50 = 145.0 nM 6.84
CHEMBL4878905 IC50 = 213.0 nM 6.67
CHEMBL4851654 IC50 = 217.0 nM 6.66
CHEMBL4856886 IC50 = 648.0 nM 6.19
CHEMBL4868547 IC50 = 2700.0 nM 5.57
CHEMBL4869822 IC50 = 4170.0 nM 5.38
CHEMBL4856096 IC50 > 100000.0 nM -
CHEMBL4875995 IC50 > 100000.0 nM -
CHEMBL4859787 IC50 > 30000.0 nM -