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Assay Detail

CHEMBL4810314

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of SARS-CoV-2 3CLpro preincubated for 30 mins followed by addition of (Dabcyl)KTSAVLQSGFRKM(Glu) peptide substrate and measured after 1.5 hrs by FRET assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Severe acute respiratory syndrome coronavirus 2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Replicase polyprotein 1ab (CHEMBL4523582)
Type SINGLE PROTEIN
Organism Severe acute respiratory syndrome coronavirus 2

Publication

A head-to-head comparison of the inhibitory activities of 15 peptidomimetic SARS-CoV-2 3CLpro inhibitors.
Vankadara S, Wong YX, Liu B, See YY, Tan LH, Tan QW, Wang G, Karuna R, Guo X, Tan ST, Fong JY, Joy J, Chia CSB.
Bioorg Med Chem Lett (2021) 48 : 128263 -128263
PubMed 34271072 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 7.47 8.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4777223 PF-00835231 -1.0 1 8.10
CHEMBL4852855 1 8.05
CHEMBL4796938 1 7.85
CHEMBL4764751 1 7.64
CHEMBL3559293 1 7.47
CHEMBL4850298 1 7.38
CHEMBL4857345 1 7.19
CHEMBL2316746 1 7.03
CHEMBL213543 1 6.54
CHEMBL2402501 1 -
CHEMBL4868590 1 -
CHEMBL92708 CALPEPTIN 1 -
CHEMBL4518490 1 -
CHEMBL64925 MG-132 1 -
CHEMBL231813 TELAPREVIR 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4777223 PF-00835231 IC50 = 8.0 nM 8.10
CHEMBL4852855 IC50 = 9.0 nM 8.05
CHEMBL4796938 IC50 = 14.0 nM 7.85
CHEMBL4764751 IC50 = 23.0 nM 7.64
CHEMBL3559293 IC50 = 34.0 nM 7.47
CHEMBL4850298 IC50 = 42.0 nM 7.38
CHEMBL4857345 IC50 = 65.0 nM 7.19
CHEMBL2316746 IC50 = 94.0 nM 7.03
CHEMBL213543 IC50 = 286.0 nM 6.54
CHEMBL2402501 IC50 > 10000.0 nM -
CHEMBL4868590 IC50 > 10000.0 nM -
CHEMBL92708 CALPEPTIN IC50 > 10000.0 nM -
CHEMBL4518490 IC50 > 10000.0 nM -
CHEMBL64925 MG-132 IC50 > 10000.0 nM -
CHEMBL231813 TELAPREVIR IC50 > 10000.0 nM -