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Assay Detail

CHEMBL4810682

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Anti-cryptosporidial activity against Cryptosporidium parvum infected in HCT-8 cells pre-infected for 3 hrs followed by compound treatment and measured after 45 hrs by fluorescence microscopy analysis
31
Total Activities
31
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Cryptosporidium parvum
Cell Type HCT-8
Confidence 1 — Organism
Curated By Autocuration

Target

Cryptosporidium parvum (CHEMBL612855)
Type ORGANISM
Organism Cryptosporidium parvum

Publication

Optimization of the Urea Linker of Triazolopyridazine MMV665917 Results in a New Anticryptosporidial Lead with Improved Potency and Predicted hERG Safety Margin.
Oboh E, Schubert TJ, Teixeira JE, Stebbins EE, Miller P, Philo E, Thakellapalli H, Campbell SD, Griggs DW, Huston CD, Meyers MJ.
J Med Chem (2021) 64 : 11729 -11745
PubMed 34342443 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 31 5.31 6.77

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4850904 1 6.77
CHEMBL4868942 1 6.26
CHEMBL4853022 1 6.21
CHEMBL4856541 1 6.18
CHEMBL4863029 1 6.07
CHEMBL1401 NITAZOXANIDE 4.0 1 5.70
CHEMBL582486 1 5.68
CHEMBL4872325 1 5.66
CHEMBL4866251 1 5.64
CHEMBL4866023 1 5.60
CHEMBL4863901 1 5.46
CHEMBL4865724 1 5.16
CHEMBL4859288 1 5.15
CHEMBL4849356 1 5.07
CHEMBL4874319 1 4.96
CHEMBL4861032 1 4.96
CHEMBL4861870 1 4.92
CHEMBL4866690 1 4.92
CHEMBL4853737 1 4.85
CHEMBL4859801 1 4.80
CHEMBL4871201 1 4.77
CHEMBL4849755 1 4.77
CHEMBL4857487 1 4.75
CHEMBL4871763 1 4.66
CHEMBL4867564 1 4.54
CHEMBL4849696 1 4.50
CHEMBL4855320 1 -
CHEMBL4855053 1 -
CHEMBL4869919 1 -
CHEMBL4863982 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4850904 EC50 = 170.0 nM 6.77
CHEMBL4868942 EC50 = 550.0 nM 6.26
CHEMBL4853022 EC50 = 610.0 nM 6.21
CHEMBL4856541 EC50 = 660.0 nM 6.18
CHEMBL4863029 EC50 = 860.0 nM 6.07
CHEMBL1401 NITAZOXANIDE EC50 = 2000.0 nM 5.70
CHEMBL582486 EC50 = 2100.0 nM 5.68
CHEMBL4872325 EC50 = 2200.0 nM 5.66
CHEMBL4866251 EC50 = 2300.0 nM 5.64
CHEMBL4866023 EC50 = 2500.0 nM 5.60
CHEMBL4863901 EC50 = 3500.0 nM 5.46
CHEMBL4865724 EC50 = 7000.0 nM 5.16
CHEMBL4859288 EC50 = 7100.0 nM 5.15
CHEMBL4849356 EC50 = 8500.0 nM 5.07
CHEMBL4874319 EC50 = 11000.0 nM 4.96
CHEMBL4861032 EC50 = 11000.0 nM 4.96
CHEMBL4861870 EC50 = 12000.0 nM 4.92
CHEMBL4866690 EC50 = 12000.0 nM 4.92
CHEMBL4853737 EC50 = 14000.0 nM 4.85
CHEMBL4859801 EC50 = 16000.0 nM 4.80
CHEMBL4871201 EC50 = 17000.0 nM 4.77
CHEMBL4849755 EC50 = 17100.0 nM 4.77
CHEMBL4857487 EC50 = 18000.0 nM 4.75
CHEMBL4871763 EC50 = 22000.0 nM 4.66
CHEMBL4867564 EC50 = 29000.0 nM 4.54
CHEMBL4849696 EC50 = 32000.0 nM 4.50
CHEMBL4855320 EC50 > 25000.0 nM -
CHEMBL4855053 EC50 > 25000.0 nM -
CHEMBL4869919 EC50 > 25000.0 nM -
CHEMBL4863982 EC50 > 25000.0 nM -
CHEMBL4855791 EC50 > 25000.0 nM -