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Assay Detail

CHEMBL4814802

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at mouse FLAG-tagged delta opioid receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP production preincubated for 20 mins followed by forskolin stimulation and measured after 15 mins by Glo-sensor reagent based assay
11
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Delta-type opioid receptor (CHEMBL3222)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Isolation and Pharmacological Characterization of Six Opioidergic <i>Picralima nitida</i> Alkaloids.
Creed SM, Gutridge AM, Argade MD, Hennessy MR, Friesen JB, Pauli GF, van Rijn RM, Riley AP.
J Nat Prod (2021) 84 : 71 -80
PubMed 33326237 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 5.44 8.90

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL8234 ENKEPHALIN 2 8.90
CHEMBL508955 AKUAMMICINE 2 4.91
CHEMBL4856018 2 4.81
CHEMBL4871880 2 4.70
CHEMBL4874877 2 4.61
CHEMBL4846243 1 4.02

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL8234 ENKEPHALIN IC50 = 1.259 nM 8.90
CHEMBL8234 ENKEPHALIN IC50 = 1.4 nM 8.85
CHEMBL508955 AKUAMMICINE IC50 = 12400.0 nM 4.91
CHEMBL508955 AKUAMMICINE IC50 = 12589.25 nM 4.90
CHEMBL4856018 IC50 = 15400.0 nM 4.81
CHEMBL4856018 IC50 = 15848.93 nM 4.80
CHEMBL4871880 IC50 = 19952.62 nM 4.70
CHEMBL4871880 IC50 = 20300.0 nM 4.69
CHEMBL4874877 IC50 = 24400.0 nM 4.61
CHEMBL4874877 IC50 = 25118.86 nM 4.60
CHEMBL4846243 IC50 = 95600.0 nM 4.02