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Assay Detail

CHEMBL4828687

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human N-terminal FLAG-tagged HDAC10 (1 to 613 residues) expressed in Sf9 insect cells using Fluor de Lys deacetylase or Fluor de Lys Green deacetylase substrate by fluorescent microplate reader assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Polyamine deacetylase HDAC10 (CHEMBL5103)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Role of Fluorination in the Histone Deacetylase 6 (HDAC6) Selectivity of Benzohydroxamate-Based Inhibitors.
Sandrone G, Cukier CD, Zrubek K, Marchini M, Vergani B, Caprini G, Fossati G, Steinkühler C, Stevenazzi A.
ACS Med Chem Lett (2021) 12 : 1810 -1817
PubMed 34795871 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 5.55 6.32

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4453121 1 6.32
CHEMBL4448410 1 5.77
CHEMBL4862563 1 5.06
CHEMBL4470373 1 5.04

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4453121 IC50 = 474.0 nM 6.32
CHEMBL4448410 IC50 = 1695.0 nM 5.77
CHEMBL4862563 IC50 = 8740.0 nM 5.06
CHEMBL4470373 IC50 = 9078.0 nM 5.04