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Assay Detail

CHEMBL4831258

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of His-tagged human AKT1 expressed in baculovirus expression system using TC-AHA-GRPRTSSFAEG-NH2 as substrate incubated for 90 mins by mobility shift assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

RAC-alpha serine/threonine-protein kinase (CHEMBL4282)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of Clinical Candidate M2698, a Dual p70S6K and Akt Inhibitor, for Treatment of PAM Pathway-Altered Cancers.
DeSelm L, Huck B, Lan R, Neagu C, Potnick J, Xiao Y, Chen X, Jones R, Richardson TE, Heasley BH, Haxell T, Moore J, Tian H, Georgi K, Rohdich F, Sutton A, Johnson T, Mochalkin I, Jackson J, Lin J, Crowley L, Machl A, Clark A, Wilker E, Sherer B, Goutopoulos A.
J Med Chem (2021) 64 : 14603 -14619
PubMed 34596404 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 7.67 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4871106 RUPITASERTIB 2.0 1 8.40
CHEMBL4860970 1 8.40
CHEMBL3685329 1 8.40
CHEMBL4876446 1 8.22
CHEMBL4846712 1 8.22
CHEMBL4856439 1 8.00
CHEMBL4855657 1 7.85
CHEMBL3675415 1 7.82
CHEMBL4871853 1 7.77
CHEMBL4851020 1 7.66
CHEMBL4875416 1 7.64
CHEMBL4854144 1 7.36
CHEMBL4869229 1 6.67
CHEMBL4853030 1 6.40
CHEMBL4857965 1 6.25
CHEMBL4853114 1 -
CHEMBL3675367 1 -
CHEMBL3675353 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4871106 RUPITASERTIB IC50 = 4.0 nM 8.40
CHEMBL4860970 IC50 = 4.0 nM 8.40
CHEMBL3685329 IC50 = 4.0 nM 8.40
CHEMBL4876446 IC50 = 6.0 nM 8.22
CHEMBL4846712 IC50 = 6.0 nM 8.22
CHEMBL4856439 IC50 = 10.0 nM 8.00
CHEMBL4855657 IC50 = 14.0 nM 7.85
CHEMBL3675415 IC50 = 15.0 nM 7.82
CHEMBL4871853 IC50 = 17.0 nM 7.77
CHEMBL4851020 IC50 = 22.0 nM 7.66
CHEMBL4875416 IC50 = 23.0 nM 7.64
CHEMBL4854144 IC50 = 44.0 nM 7.36
CHEMBL4869229 IC50 = 212.0 nM 6.67
CHEMBL4853030 IC50 = 400.0 nM 6.40
CHEMBL4857965 IC50 = 563.0 nM 6.25
CHEMBL4853114 IC50 > 10000.0 nM -
CHEMBL3675367 IC50 > 10000.0 nM -
CHEMBL3675353 IC50 > 10000.0 nM -