Assay Detail
Binding
CHEMBL4831260
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant full length GST-tagged human Aurora B (1 to 344 residues) co-expressed with N-terminal His-tagged human recombinant INCENP (803 to 918 residues) in baculovirus infected Sf21 cells incubated for 90 mins by mobility shift assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Sf21 |
| Confidence | 7 — Homologous single protein target |
| Curated By | Autocuration |
Target
Aurora kinase B/Inner centromere protein (CHEMBL3430907) ↗| Type | PROTEIN COMPLEX |
| Organism | Homo sapiens |
Publication
Identification of Clinical Candidate M2698, a Dual p70S6K and Akt Inhibitor, for Treatment of PAM Pathway-Altered Cancers.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 15 | 7.32 | 8.40 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3685329 | — | — | 1 | 8.40 |
| CHEMBL4854144 | — | — | 1 | 8.22 |
| CHEMBL3675415 | — | — | 1 | 8.05 |
| CHEMBL4875416 | — | — | 1 | 7.39 |
| CHEMBL4851020 | — | — | 1 | 7.35 |
| CHEMBL4857965 | — | — | 1 | 7.35 |
| CHEMBL4855657 | — | — | 1 | 7.33 |
| CHEMBL4869229 | — | — | 1 | 7.29 |
| CHEMBL4860970 | — | — | 1 | 7.25 |
| CHEMBL4856439 | — | — | 1 | 7.12 |
| CHEMBL4871106 | RUPITASERTIB | 2.0 | 1 | 6.77 |
| CHEMBL4876446 | — | — | 1 | 6.75 |
| CHEMBL4846712 | — | — | 1 | 6.63 |
| CHEMBL4871853 | — | — | 1 | 6.58 |
| CHEMBL4853030 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3685329 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL4854144 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL3675415 | — | IC50 | = | 9.0 | nM | 8.05 |
| CHEMBL4875416 | — | IC50 | = | 41.0 | nM | 7.39 |
| CHEMBL4851020 | — | IC50 | = | 45.0 | nM | 7.35 |
| CHEMBL4857965 | — | IC50 | = | 45.0 | nM | 7.35 |
| CHEMBL4855657 | — | IC50 | = | 47.0 | nM | 7.33 |
| CHEMBL4869229 | — | IC50 | = | 51.0 | nM | 7.29 |
| CHEMBL4860970 | — | IC50 | = | 56.0 | nM | 7.25 |
| CHEMBL4856439 | — | IC50 | = | 75.0 | nM | 7.12 |
| CHEMBL4871106 | RUPITASERTIB | IC50 | = | 170.0 | nM | 6.77 |
| CHEMBL4876446 | — | IC50 | = | 180.0 | nM | 6.75 |
| CHEMBL4846712 | — | IC50 | = | 233.0 | nM | 6.63 |
| CHEMBL4871853 | — | IC50 | = | 260.0 | nM | 6.58 |
| CHEMBL4853030 | — | IC50 | > | 1000.0 | nM | - |