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Assay Detail

CHEMBL4834853

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at human MT1 receptor expressed in CHO cells assessed as increase in cAMP levels incubated at 28 degreeC by cellular dielectric spectroscopic analysis
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Melatonin receptor type 1A (CHEMBL1945)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of <b>ACH-000143</b>: A Novel Potent and Peripherally Preferred Melatonin Receptor Agonist that Reduces Liver Triglycerides and Steatosis in Diet-Induced Obese Rats.
Ferreira MA, Azevedo H, Mascarello A, Segretti ND, Russo E, Russo V, Guimarães CRW.
J Med Chem (2021) 64 : 1904 -1929
PubMed 33626870 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 23 9.38 9.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL45 MELATONIN 4.0 1 9.96
CHEMBL4874954 1 9.85
CHEMBL4852096 1 9.85
CHEMBL4848885 1 9.74
CHEMBL4854625 1 9.70
CHEMBL4867553 1 9.62
CHEMBL4859295 1 9.60
CHEMBL4852440 1 9.49
CHEMBL4859677 1 9.48
CHEMBL4873903 1 9.38
CHEMBL4869463 1 9.12
CHEMBL4872217 1 9.01
CHEMBL4854836 1 8.72
CHEMBL4846505 1 8.67
CHEMBL4860729 1 8.45
CHEMBL4875780 1 -
CHEMBL4861281 1 -
CHEMBL4849193 1 -
CHEMBL4867364 1 -
CHEMBL4867474 1 -
CHEMBL4854434 1 -
CHEMBL4867993 1 -
CHEMBL113692 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL45 MELATONIN EC50 = 0.11 nM 9.96
CHEMBL4874954 EC50 = 0.14 nM 9.85
CHEMBL4852096 EC50 = 0.14 nM 9.85
CHEMBL4848885 EC50 = 0.18 nM 9.74
CHEMBL4854625 EC50 = 0.2 nM 9.70
CHEMBL4867553 EC50 = 0.24 nM 9.62
CHEMBL4859295 EC50 = 0.25 nM 9.60
CHEMBL4852440 EC50 = 0.32 nM 9.49
CHEMBL4859677 EC50 = 0.33 nM 9.48
CHEMBL4873903 EC50 = 0.42 nM 9.38
CHEMBL4869463 EC50 = 0.75 nM 9.12
CHEMBL4872217 EC50 = 0.97 nM 9.01
CHEMBL4854836 EC50 = 1.92 nM 8.72
CHEMBL4846505 EC50 = 2.13 nM 8.67
CHEMBL4860729 EC50 = 3.58 nM 8.45
CHEMBL4875780 EC50 - -
CHEMBL4861281 EC50 - -
CHEMBL4849193 EC50 - -
CHEMBL4867364 EC50 - -
CHEMBL4867474 EC50 - -
CHEMBL4854434 EC50 - -
CHEMBL4867993 EC50 - -
CHEMBL113692 EC50 - -