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Assay Detail

CHEMBL4835153

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of 2-[125l]-lodomelatonin from human MT1 expressed in CHO cell membrane incubated for 150 mins by radioligand binding assay
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Subcellular Cell membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Melatonin receptor type 1A (CHEMBL1945)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a Potent and Orally Bioavailable Melatonin Receptor Agonist.
Hoashi Y, Takai T, Kosugi Y, Nakashima M, Nakayama M, Hirai K, Uchikawa O, Koike T.
J Med Chem (2021) 64 : 3059 -3074
PubMed 33682410 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 19 9.45 10.57

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4868554 1 10.57
CHEMBL3648356 1 10.51
CHEMBL3648362 1 10.43
CHEMBL3648355 1 10.17
CHEMBL3648358 1 10.17
CHEMBL4874630 1 9.77
CHEMBL4874010 1 9.70
CHEMBL3648365 1 9.66
CHEMBL45 MELATONIN 4.0 1 9.62
CHEMBL4869029 1 9.52
CHEMBL4872592 1 9.46
CHEMBL3648366 1 9.41
CHEMBL3648367 1 9.35
CHEMBL3648354 1 9.35
CHEMBL4848381 1 8.92
CHEMBL4864102 1 8.89
CHEMBL4870108 1 8.36
CHEMBL4871181 1 8.03
CHEMBL3648357 1 7.64

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4868554 Ki = 0.027 nM 10.57
CHEMBL3648356 Ki = 0.031 nM 10.51
CHEMBL3648362 Ki = 0.037 nM 10.43
CHEMBL3648355 Ki = 0.067 nM 10.17
CHEMBL3648358 Ki = 0.067 nM 10.17
CHEMBL4874630 Ki = 0.17 nM 9.77
CHEMBL4874010 Ki = 0.2 nM 9.70
CHEMBL3648365 Ki = 0.22 nM 9.66
CHEMBL45 MELATONIN Ki = 0.24 nM 9.62
CHEMBL4869029 Ki = 0.3 nM 9.52
CHEMBL4872592 Ki = 0.35 nM 9.46
CHEMBL3648366 Ki = 0.39 nM 9.41
CHEMBL3648367 Ki = 0.45 nM 9.35
CHEMBL3648354 Ki = 0.45 nM 9.35
CHEMBL4848381 Ki = 1.2 nM 8.92
CHEMBL4864102 Ki = 1.3 nM 8.89
CHEMBL4870108 Ki = 4.4 nM 8.36
CHEMBL4871181 Ki = 9.3 nM 8.03
CHEMBL3648357 Ki = 23.0 nM 7.64