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Assay Detail

CHEMBL4835155

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of 2-[125l]-lodomelatonin from human MT2 expressed in CHO cell membrane incubated for 150 mins by radioligand binding assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Subcellular Cell membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Melatonin receptor type 1B (CHEMBL1946)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a Potent and Orally Bioavailable Melatonin Receptor Agonist.
Hoashi Y, Takai T, Kosugi Y, Nakashima M, Nakayama M, Hirai K, Uchikawa O, Koike T.
J Med Chem (2021) 64 : 3059 -3074
PubMed 33682410 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 18 9.60 10.24

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4868554 1 10.24
CHEMBL3648356 1 10.15
CHEMBL3648354 1 10.14
CHEMBL3648362 1 10.07
CHEMBL3648358 1 10.06
CHEMBL4870108 1 9.92
CHEMBL3648355 1 9.89
CHEMBL3648367 1 9.82
CHEMBL3648366 1 9.82
CHEMBL3648365 1 9.80
CHEMBL45 MELATONIN 4.0 1 9.68
CHEMBL4864102 1 9.64
CHEMBL4869029 1 9.46
CHEMBL4872592 1 9.12
CHEMBL4874630 1 9.02
CHEMBL4848381 1 8.96
CHEMBL4874010 1 8.85
CHEMBL3648357 1 8.21

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4868554 Ki = 0.058 nM 10.24
CHEMBL3648356 Ki = 0.07 nM 10.15
CHEMBL3648354 Ki = 0.073 nM 10.14
CHEMBL3648362 Ki = 0.086 nM 10.07
CHEMBL3648358 Ki = 0.088 nM 10.06
CHEMBL4870108 Ki = 0.12 nM 9.92
CHEMBL3648355 Ki = 0.13 nM 9.89
CHEMBL3648367 Ki = 0.15 nM 9.82
CHEMBL3648366 Ki = 0.15 nM 9.82
CHEMBL3648365 Ki = 0.16 nM 9.80
CHEMBL45 MELATONIN Ki = 0.21 nM 9.68
CHEMBL4864102 Ki = 0.23 nM 9.64
CHEMBL4869029 Ki = 0.35 nM 9.46
CHEMBL4872592 Ki = 0.75 nM 9.12
CHEMBL4874630 Ki = 0.95 nM 9.02
CHEMBL4848381 Ki = 1.1 nM 8.96
CHEMBL4874010 Ki = 1.4 nM 8.85
CHEMBL3648357 Ki = 6.1 nM 8.21