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Assay Detail

CHEMBL4839446

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of NanoLuc-fused SLK (unknown origin) expressed in HEK293T cells after 2 hrs by NanoBRET assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293T
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

STE20-like serine/threonine-protein kinase (CHEMBL4202)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a Potent Dual SLK/STK10 Inhibitor Based on a Maleimide Scaffold.
Serafim RAM, Sorrell FJ, Berger BT, Collins RJ, Vasconcelos SNS, Massirer KB, Knapp S, Bennett J, Fedorov O, Patel H, Zuercher WJ, Elkins JM.
J Med Chem (2021) 64 : 13259 -13278
PubMed 34463505 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 5.39 6.16

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL156277 1 6.16
CHEMBL4875188 1 5.92
CHEMBL4878325 1 4.77
CHEMBL4856751 1 4.72
CHEMBL4877889 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL156277 IC50 = 700.0 nM 6.16
CHEMBL4875188 IC50 = 1200.0 nM 5.92
CHEMBL4878325 IC50 = 17000.0 nM 4.77
CHEMBL4856751 IC50 = 19000.0 nM 4.72
CHEMBL4877889 IC50 > 20000.0 nM -