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Assay Detail

CHEMBL4840200

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at glucocorticoid receptor in rat INS-1 832/13 cells transfected with CCL2-promoter luciferase plasmid construct assessed as reduction in IL-1 beta induced inflammation by luciferase reporter gene assay
34
Total Activities
34
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Cell Type INS-1 832/13
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Glucocorticoid receptor (CHEMBL3368)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Potent Anti-Inflammatory, Arylpyrazole-Based Glucocorticoid Receptor Agonists That Do Not Impair Insulin Secretion.
Kennedy BJ, Lato AM, Fisch AR, Burke SJ, Kirkland JK, Prevatte CW, Dunlap LE, Smith RT, Vogiatzis KD, Collier JJ, Campagna SR.
ACS Med Chem Lett (2021) 12 : 1568 -1577
PubMed 34676039 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 34 7.42 9.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4863316 1 9.80
CHEMBL4877227 1 9.70
CHEMBL312627 1 9.60
CHEMBL4850526 1 8.70
CHEMBL4857046 1 8.50
CHEMBL311662 1 8.30
CHEMBL233390 1 8.30
CHEMBL384467 DEXAMETHASONE 4.0 1 8.20
CHEMBL420767 1 8.20
CHEMBL4872787 1 8.20
CHEMBL4855992 1 8.10
CHEMBL4868414 1 8.10
CHEMBL79807 1 8.00
CHEMBL313062 1 7.80
CHEMBL389621 HYDROCORTISONE 4.0 1 7.60
CHEMBL4846557 1 7.50
CHEMBL4875267 1 7.40
CHEMBL81591 1 7.40
CHEMBL312041 1 7.40
CHEMBL4853394 1 7.20
CHEMBL4856145 1 7.10
CHEMBL4872009 1 7.00
CHEMBL4871954 1 6.80
CHEMBL4866467 1 6.60
CHEMBL4867686 1 6.60
CHEMBL4860401 1 6.40
CHEMBL4864367 1 6.30
CHEMBL4876447 1 6.30
CHEMBL4850324 1 6.20
CHEMBL4845663 1 5.90

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4863316 IC50 = 0.1585 nM 9.80
CHEMBL4877227 IC50 = 0.1995 nM 9.70
CHEMBL312627 IC50 = 0.2512 nM 9.60
CHEMBL4850526 IC50 = 1.995 nM 8.70
CHEMBL4857046 IC50 = 3.162 nM 8.50
CHEMBL311662 IC50 = 5.012 nM 8.30
CHEMBL233390 IC50 = 5.012 nM 8.30
CHEMBL384467 DEXAMETHASONE IC50 = 6.31 nM 8.20
CHEMBL420767 IC50 = 6.31 nM 8.20
CHEMBL4872787 IC50 = 6.31 nM 8.20
CHEMBL4855992 IC50 = 7.943 nM 8.10
CHEMBL4868414 IC50 = 7.943 nM 8.10
CHEMBL79807 IC50 = 10.0 nM 8.00
CHEMBL313062 IC50 = 15.85 nM 7.80
CHEMBL389621 HYDROCORTISONE IC50 = 25.12 nM 7.60
CHEMBL4846557 IC50 = 31.62 nM 7.50
CHEMBL312041 IC50 = 39.81 nM 7.40
CHEMBL81591 IC50 = 39.81 nM 7.40
CHEMBL4875267 IC50 = 39.81 nM 7.40
CHEMBL4853394 IC50 = 63.1 nM 7.20
CHEMBL4856145 IC50 = 79.43 nM 7.10
CHEMBL4872009 IC50 = 100.0 nM 7.00
CHEMBL4871954 IC50 = 158.49 nM 6.80
CHEMBL4866467 IC50 = 251.19 nM 6.60
CHEMBL4867686 IC50 = 251.19 nM 6.60
CHEMBL4860401 IC50 = 398.11 nM 6.40
CHEMBL4864367 IC50 = 501.19 nM 6.30
CHEMBL4876447 IC50 = 501.19 nM 6.30
CHEMBL4850324 IC50 = 630.96 nM 6.20
CHEMBL4845663 IC50 = 1258.93 nM 5.90
CHEMBL4868293 IC50 = 1258.93 nM 5.90
CHEMBL4871994 IC50 = 1584.89 nM 5.80
CHEMBL4867877 IC50 = 1584.89 nM 5.80
CHEMBL4850604 IC50 = 2511.89 nM 5.60