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Assay Detail

CHEMBL4840248

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Functional
Agonist activity at rat APJ receptor expressed in HEK293T cells assessed as compound stimulated inhibition of forskolin stimulated cAMP production incubated for 30 mins by HTRF assay
22
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Rattus norvegicus
Cell Type HEK293T
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Apelin receptor (CHEMBL2398)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Identification of 6-Hydroxypyrimidin-4(1<i>H</i>)-one-3-carboxamides as Potent and Orally Active APJ Receptor Agonists.
Pi Z, Johnson JA, Meng W, Phillips M, Schumacher WA, Bostwick JS, Gargalovic PS, Onorato JM, Generaux CN, Wang T, He Y, Gordon DA, Wexler RR, Finlay HJ.
ACS Med Chem Lett (2021) 12 : 1766 -1772
PubMed 34795866 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 22 8.93 10.62

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4861895 1 10.62
CHEMBL4854412 1 10.46
CHEMBL4867145 1 10.20
CHEMBL3184840 1 10.15
CHEMBL4874120 2 9.82
CHEMBL4854048 2 9.77
CHEMBL4855254 1 9.57
CHEMBL4863297 2 9.48
CHEMBL4872407 1 9.47
CHEMBL4870497 1 9.21
CHEMBL4851693 1 8.74
CHEMBL4875108 2 8.68
CHEMBL4869091 1 8.51
CHEMBL4856612 1 8.00
CHEMBL4845664 1 6.82
CHEMBL4854830 1 5.92
CHEMBL4848469 1 5.32
CHEMBL4863722 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4861895 EC50 = 0.024 nM 10.62
CHEMBL4854412 EC50 = 0.035 nM 10.46
CHEMBL4867145 EC50 = 0.063 nM 10.20
CHEMBL3184840 EC50 = 0.07 nM 10.15
CHEMBL4874120 EC50 = 0.15 nM 9.82
CHEMBL4854048 EC50 = 0.17 nM 9.77
CHEMBL4855254 EC50 = 0.27 nM 9.57
CHEMBL4854048 EC50 = 0.31 nM 9.51
CHEMBL4863297 EC50 = 0.33 nM 9.48
CHEMBL4872407 EC50 = 0.34 nM 9.47
CHEMBL4874120 EC50 = 0.37 nM 9.43
CHEMBL4863297 EC50 = 0.4 nM 9.40
CHEMBL4870497 EC50 = 0.61 nM 9.21
CHEMBL4851693 EC50 = 1.8 nM 8.74
CHEMBL4875108 EC50 = 2.1 nM 8.68
CHEMBL4869091 EC50 = 3.1 nM 8.51
CHEMBL4875108 EC50 = 4.0 nM 8.40
CHEMBL4856612 EC50 = 10.0 nM 8.00
CHEMBL4845664 EC50 = 150.0 nM 6.82
CHEMBL4854830 EC50 = 1200.0 nM 5.92
CHEMBL4848469 EC50 = 4800.0 nM 5.32
CHEMBL4863722 EC50 > 30000.0 nM -