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Assay Detail

CHEMBL5034977

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [125I]-NDP-alpha-MSH from human MC4R expressed in HEK2936E cell membrane measured after 16-23 hrs by 1450 microbeta trilux scintillation proximity assay
28
Total Activities
28
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK2936E
Subcellular Cell membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Melanocortin receptor 4 (CHEMBL259)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Melanocortin 1 Receptor Agonists Based on a Bivalent, Bicyclic Peptide Framework.
Durek T, Kaas Q, White AM, Weidmann J, Fuaad AA, Cheneval O, Schroeder CI, de Veer SJ, Dellsén A, Österlund T, Larsson N, Knerr L, Bauer U, Plowright AT, Craik DJ.
J Med Chem (2021) 64 : 9906 -9915
PubMed 34197114 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 28 7.31 8.90

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL430239 MELATONAN 1 8.90
CHEMBL3301624 SETMELANOTIDE 4.0 1 8.39
CHEMBL5076315 1 8.10
CHEMBL5087839 1 8.09
CHEMBL5090670 1 7.99
CHEMBL5090285 1 7.97
CHEMBL5083551 1 7.87
CHEMBL5080489 1 7.82
CHEMBL2070241 BREMELANOTIDE 4.0 1 7.66
CHEMBL5077095 1 7.59
CHEMBL5090946 1 7.59
CHEMBL5087814 1 7.52
CHEMBL5087859 1 7.52
CHEMBL5080784 1 7.44
CHEMBL5077144 1 7.30
CHEMBL5094168 1 7.19
CHEMBL5094215 1 7.14
CHEMBL5078687 1 7.04
CHEMBL5075712 1 6.94
CHEMBL5091236 1 6.86
CHEMBL5081077 1 6.80
CHEMBL5091245 1 6.51
CHEMBL5085972 1 6.46
CHEMBL5092761 1 6.45
CHEMBL5075506 1 6.23
CHEMBL4099889 1 6.21
CHEMBL5077811 1 5.69
CHEMBL5093939 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL430239 MELATONAN Ki = 1.259 nM 8.90
CHEMBL3301624 SETMELANOTIDE Ki = 4.074 nM 8.39
CHEMBL5076315 Ki = 7.943 nM 8.10
CHEMBL5087839 Ki = 8.128 nM 8.09
CHEMBL5090670 Ki = 10.23 nM 7.99
CHEMBL5090285 Ki = 10.72 nM 7.97
CHEMBL5083551 Ki = 13.49 nM 7.87
CHEMBL5080489 Ki = 15.14 nM 7.82
CHEMBL2070241 BREMELANOTIDE Ki = 21.88 nM 7.66
CHEMBL5077095 Ki = 25.7 nM 7.59
CHEMBL5090946 Ki = 25.7 nM 7.59
CHEMBL5087814 Ki = 30.2 nM 7.52
CHEMBL5087859 Ki = 30.2 nM 7.52
CHEMBL5080784 Ki = 36.31 nM 7.44
CHEMBL5077144 Ki = 50.12 nM 7.30
CHEMBL5094168 Ki = 64.57 nM 7.19
CHEMBL5094215 Ki = 72.44 nM 7.14
CHEMBL5078687 Ki = 91.2 nM 7.04
CHEMBL5075712 Ki = 114.82 nM 6.94
CHEMBL5091236 Ki = 138.04 nM 6.86
CHEMBL5081077 Ki = 158.49 nM 6.80
CHEMBL5091245 Ki = 309.03 nM 6.51
CHEMBL5085972 Ki = 346.74 nM 6.46
CHEMBL5092761 Ki = 354.81 nM 6.45
CHEMBL5075506 Ki = 588.84 nM 6.23
CHEMBL4099889 Ki = 616.6 nM 6.21
CHEMBL5077811 Ki = 2041.74 nM 5.69
CHEMBL5093939 Ki > 1000.0 nM -