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Assay Detail

CHEMBL5038093

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of horse serum BChE incubated for 5 mins by DTNB reagent based Ellman's method
21
Total Activities
11
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Equus caballus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cholinesterase (CHEMBL5763)
Type SINGLE PROTEIN
Organism Equus caballus

Publication

Spatial Distribution and Stability of Cholinesterase Inhibitory Protoberberine Alkaloids from <i>Papaver setiferum</i>.
Safa N, Trobec T, Holland DC, Slazak B, Jacobsson E, Hawkes JA, Frangež R, Sepčić K, Göransson U, Moodie LWK, Robertson LP.
J Nat Prod (2022) 85 : 215 -224
PubMed 34910498 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 4.49 5.55
Ki 10 4.88 6.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5091026 2 6.22
CHEMBL4922390 2 5.66
CHEMBL5085317 2 4.45
CHEMBL5080190 2 4.36
CHEMBL5076804 2 4.33
CHEMBL5089821 2 4.23
CHEMBL211471 NEOSTIGMINE METHYLSULFATE 4.0 1 4.03
CHEMBL5080442 2 -
CHEMBL5087459 2 -
CHEMBL404097 SALUTARIDINE 2 -
CHEMBL5077034 2 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5091026 Ki = 600.0 nM 6.22
CHEMBL4922390 Ki = 2200.0 nM 5.66
CHEMBL5091026 IC50 = 2800.0 nM 5.55
CHEMBL4922390 IC50 = 7100.0 nM 5.15
CHEMBL5085317 Ki = 35500.0 nM 4.45
CHEMBL5080190 Ki = 43700.0 nM 4.36
CHEMBL5076804 Ki = 47000.0 nM 4.33
CHEMBL5089821 Ki = 58500.0 nM 4.23
CHEMBL5080190 IC50 = 63100.0 nM 4.20
CHEMBL211471 NEOSTIGMINE METHYLSULFATE IC50 = 92700.0 nM 4.03
CHEMBL5076804 IC50 = 98500.0 nM 4.01
CHEMBL5089821 IC50 = 100000.0 nM 4.00
CHEMBL5077034 Ki - -
CHEMBL5087459 IC50 = 221000.0 nM -
CHEMBL5077034 IC50 = 342000.0 nM -
CHEMBL5080442 IC50 - -
CHEMBL5085317 IC50 = 104000.0 nM -
CHEMBL5080442 Ki - -
CHEMBL404097 SALUTARIDINE Ki - -
CHEMBL404097 SALUTARIDINE IC50 = 335000.0 nM -
CHEMBL5087459 Ki - -