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Assay Detail

CHEMBL5054300

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human BRD4 (1 to 477 residues) measured after 60 mins by TR-FRET assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bromodomain-containing protein 4 (CHEMBL1163125)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery and Preclinical Pharmacology of an Oral Bromodomain and Extra-Terminal (BET) Inhibitor Using Scaffold-Hopping and Structure-Guided Drug Design.
Gavai AV, Norris D, Delucca G, Tortolani D, Tokarski JS, Dodd D, O'Malley D, Zhao Y, Quesnelle C, Gill P, Vaccaro W, Huynh T, Ahuja V, Han WC, Mussari C, Harikrishnan L, Kamau M, Poss M, Sheriff S, Yan C, Marsilio F, Menard K, Wen ML, Rampulla R, Wu DR, Li J, Zhang H, Li P, Sun D, Yip H, Traeger SC, Zhang Y, Mathur A, Zhang H, Huang C, Yang Z, Ranasinghe A, Everlof G, Raghavan N, Tye CK, Wee S, Hunt JT, Vite G, Westhouse R, Lee FY.
J Med Chem (2021) 64 : 14247 -14265
PubMed 34543572 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 7.84 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5080354 1 9.00
CHEMBL4297458 EZOBRESIB 2.0 1 8.96
CHEMBL5093440 1 8.82
CHEMBL5087175 1 8.72
CHEMBL5082474 1 8.72
CHEMBL5074438 1 8.38
CHEMBL3786449 1 8.36
CHEMBL5074708 1 8.35
CHEMBL5075046 1 8.27
CHEMBL5075119 1 8.02
CHEMBL5089900 1 7.64
CHEMBL5085106 1 7.46
CHEMBL5090216 1 7.41
CHEMBL5094725 1 7.16
CHEMBL5080617 1 6.88
CHEMBL5093519 1 6.54
CHEMBL5090264 1 6.28
CHEMBL5083442 1 6.15

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5080354 IC50 = 1.0 nM 9.00
CHEMBL4297458 EZOBRESIB IC50 = 1.1 nM 8.96
CHEMBL5093440 IC50 = 1.5 nM 8.82
CHEMBL5087175 IC50 = 1.9 nM 8.72
CHEMBL5082474 IC50 = 1.9 nM 8.72
CHEMBL5074438 IC50 = 4.2 nM 8.38
CHEMBL3786449 IC50 = 4.4 nM 8.36
CHEMBL5074708 IC50 = 4.5 nM 8.35
CHEMBL5075046 IC50 = 5.4 nM 8.27
CHEMBL5075119 IC50 = 9.5 nM 8.02
CHEMBL5089900 IC50 = 23.0 nM 7.64
CHEMBL5085106 IC50 = 35.0 nM 7.46
CHEMBL5090216 IC50 = 39.0 nM 7.41
CHEMBL5094725 IC50 = 69.0 nM 7.16
CHEMBL5080617 IC50 = 131.0 nM 6.88
CHEMBL5093519 IC50 = 292.0 nM 6.54
CHEMBL5090264 IC50 = 530.0 nM 6.28
CHEMBL5083442 IC50 = 712.0 nM 6.15