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Assay Detail

CHEMBL5055012

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]-citalopram from human SERT expressed in HEK cell membrane assessed as inhibition constant by radioligand binding assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK
Subcellular Cell membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium-dependent serotonin transporter (CHEMBL228)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

C-terminal modified Enkephalin-like tetrapeptides with enhanced affinities at the kappa opioid receptor and monoamine transporters.
Mehr-Un-Nisa, Munawar MA, Rankin D, Hruby VJ, Porreca F, Lee YS.
Bioorg Med Chem (2021) 51 : 116509 -116509
PubMed 34798381 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 8 6.01 8.04

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL629 AMITRIPTYLINE 4.0 1 8.04
CHEMBL5089012 1 6.06
CHEMBL5077561 1 6.00
CHEMBL5090705 1 5.75
CHEMBL5078669 1 5.64
CHEMBL5081245 1 5.60
CHEMBL5091642 1 4.96
CHEMBL5086370 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL629 AMITRIPTYLINE Ki = 9.2 nM 8.04
CHEMBL5089012 Ki = 880.0 nM 6.06
CHEMBL5077561 Ki = 1000.0 nM 6.00
CHEMBL5090705 Ki = 1800.0 nM 5.75
CHEMBL5078669 Ki = 2300.0 nM 5.64
CHEMBL5081245 Ki = 2500.0 nM 5.60
CHEMBL5091642 Ki = 11000.0 nM 4.96
CHEMBL5086370 Ki > 10000.0 nM -