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Assay Detail

CHEMBL5056986

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at PR in human T47D cells using p-nitrophenyl phosphate as substrate assessed as induction of alkaline phosphatase activity incubated for 72 hrs followed by substrate addition measured after 24 hrs by alkaline phosphatase assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type T47D
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Progesterone receptor (CHEMBL208)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis of Norgestomet and its 17β-isomer and evaluation of their agonistic activities against progesterone receptor.
Kurohara T, Ito T, Tsuji G, Misawa T, Yokoo H, Yanase Y, Shoda T, Sakai T, Hosoe J, Uchiyama N, Akiyama H, Demizu Y.
Bioorg Med Chem (2021) 49 : 116425 -116425
PubMed 34607200 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 3 9.05 9.74

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL103 PROGESTERONE 4.0 1 9.74
CHEMBL2104744 NORGESTOMET 2.0 1 8.35
CHEMBL5077912 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL103 PROGESTERONE EC50 = 0.18 nM 9.74
CHEMBL2104744 NORGESTOMET EC50 = 4.5 nM 8.35
CHEMBL5077912 EC50 > 125.0 nM -