Assay Detail
Binding
CHEMBL5058554
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Cellular target engagement assay with: SIK3
4
Total Activities
1
Compounds Tested
2
Activity Types
1
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | HEK-293T |
| Category | NanoBRET assay |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Serine/threonine-protein kinase SIK3 (CHEMBL6149) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Tm Shift (DSF) assay results for EUbOPEN Chemogenomis Library 2 (NanoBRET)
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| LogIC50 | 2 | - | - |
| IC50 | 2 | 8.16 | 8.22 |
Assay Parameters
| Parameter | Type | Value | Units | Text |
|---|---|---|---|---|
| Cell Line | Cell Line | - | — | HEK293T |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5090394 | — | — | 4 | 8.22 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5090394 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL5090394 | — | IC50 | = | 8.0 | nM | 8.10 |
| CHEMBL5090394 | — | LogIC50 | = | -8.0 | — | - |
| CHEMBL5090394 | — | LogIC50 | = | -8.0 | — | - |