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Assay Detail

CHEMBL5099988

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]NCS-382 from CaMK2alpha in rat brain cortical membrane homogenates measured after 1 hr by TopCount scintillation counting method
32
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Cortex
Subcellular Membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Exploring the NCS-382 Scaffold for CaMKIIα Modulation: Synthesis, Biochemical Pharmacology, and Biophysical Characterization of Ph-HTBA as a Novel High-Affinity Brain-Penetrant Stabilizer of the CaMKIIα Hub Domain.
Tian Y, Shehata MA, Gauger SJ, Veronesi C, Hamborg L, Thiesen L, Bruus-Jensen J, Royssen JS, Leurs U, Larsen ASG, Krall J, Solbak SMØ, Wellendorph P, Frølund B.
J Med Chem (2022) 65 : 15066 -15084
PubMed 36346645 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 32 6.57 7.50

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5176248 2 7.50
CHEMBL1031 5-HYDROXY-DICLOFENAC 1 7.46
CHEMBL5170394 2 7.40
CHEMBL5182530 2 7.30
CHEMBL5189628 2 7.30
CHEMBL5177983 2 7.30
CHEMBL5170093 2 7.11
CHEMBL5175633 2 7.00
CHEMBL5198432 2 7.00
CHEMBL2431324 1 6.80
CHEMBL5201182 2 6.70
CHEMBL5208394 2 6.50
CHEMBL472694 1 6.47
CHEMBL5196579 2 6.42
CHEMBL1342 OXYBATE 4.0 1 5.37
CHEMBL5196334 2 5.01
CHEMBL5183075 2 4.90
CHEMBL5182678 2 4.90

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5176248 Ki = 31.62 nM 7.50
CHEMBL1031 5-HYDROXY-DICLOFENAC Ki = 35.0 nM 7.46
CHEMBL5176248 Ki = 40.0 nM 7.40
CHEMBL5170394 Ki = 39.81 nM 7.40
CHEMBL5182530 Ki = 50.12 nM 7.30
CHEMBL5177983 Ki = 50.12 nM 7.30
CHEMBL5170394 Ki = 50.0 nM 7.30
CHEMBL5189628 Ki = 50.12 nM 7.30
CHEMBL5177983 Ki = 53.0 nM 7.28
CHEMBL5189628 Ki = 52.0 nM 7.28
CHEMBL5182530 Ki = 56.0 nM 7.25
CHEMBL5170093 Ki = 78.0 nM 7.11
CHEMBL5170093 Ki = 79.43 nM 7.10
CHEMBL5198432 Ki = 100.0 nM 7.00
CHEMBL5175633 Ki = 100.0 nM 7.00
CHEMBL5175633 Ki = 110.0 nM 6.96
CHEMBL5198432 Ki = 110.0 nM 6.96
CHEMBL2431324 Ki = 160.0 nM 6.80
CHEMBL5201182 Ki = 199.53 nM 6.70
CHEMBL5201182 Ki = 230.0 nM 6.64
CHEMBL5208394 Ki = 316.23 nM 6.50
CHEMBL5208394 Ki = 320.0 nM 6.50
CHEMBL472694 Ki = 340.0 nM 6.47
CHEMBL5196579 Ki = 380.0 nM 6.42
CHEMBL5196579 Ki = 398.11 nM 6.40
CHEMBL1342 OXYBATE Ki = 4300.0 nM 5.37
CHEMBL5196334 Ki = 9700.0 nM 5.01
CHEMBL5196334 Ki = 10000.0 nM 5.00
CHEMBL5183075 Ki = 12589.25 nM 4.90
CHEMBL5182678 Ki = 12589.25 nM 4.90
CHEMBL5183075 Ki = 14000.0 nM 4.85
CHEMBL5182678 Ki = 14000.0 nM 4.85