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Assay Detail

CHEMBL5109147

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to FITC-labelled dsDNA (unknown origin) assessed as dissociation constant incubated for 10 mins by fluorescence polarization assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Not specified
Confidence 3 — Cell-line / Tissue
Curated By Autocuration

Target

dsDNA (CHEMBL3832741)
Type NUCLEIC-ACID
Organism Homo sapiens

Publication

RpoN-Based stapled peptides with improved DNA binding suppress <i>Pseudomonas aeruginosa</i> virulence.
Paquette AR, Payne SR, McKay GA, Brazeau-Henrie JT, Darnowski MG, Kammili A, Bernal F, Mah TF, Gruenheid S, Nguyen D, Boddy CN.
RSC Med Chem (2022) 13 : 445 -455
PubMed 35647551 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 8 6.23 6.46

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5183480 1 6.46
CHEMBL5182279 1 6.44
CHEMBL5175391 1 6.42
CHEMBL5174777 1 6.42
CHEMBL5173726 1 6.08
CHEMBL5185120 1 6.02
CHEMBL5170110 1 5.78
CHEMBL5170635 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5183480 Kd = 346.0 nM 6.46
CHEMBL5182279 Kd = 366.0 nM 6.44
CHEMBL5175391 Kd = 385.0 nM 6.42
CHEMBL5174777 Kd = 376.0 nM 6.42
CHEMBL5173726 Kd = 834.0 nM 6.08
CHEMBL5185120 Kd = 947.0 nM 6.02
CHEMBL5170110 Kd = 1680.0 nM 5.78
CHEMBL5170635 Kd > 20000000.0 nM -