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Assay Detail

CHEMBL5111584

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of GCN2 in human SK-OV-3 cells assessed as reduction in phosphorylation of eIF2alpha by cellular assay
36
Total Activities
36
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type SK-OV-3
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

eIF-2-alpha kinase GCN2 (CHEMBL5358)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Potent GCN2 Inhibitor Capable of Reversing MDSC-Driven T Cell Suppression Demonstrates In Vivo Efficacy as a Single Agent and in Combination with Anti-Angiogenesis Therapy.
Jackson JJ, Shibuya GM, Ravishankar B, Adusumilli L, Bradford D, Brockstedt DG, Bucher C, Bui M, Cho C, Colas C, Cutler G, Dukes A, Han X, Hu DX, Jacobson S, Kassner PD, Katibah GE, Ko MYM, Kolhatkar U, Leger PR, Ma A, Marshall L, Maung J, Ng AA, Okano A, Pookot D, Poon D, Ramana C, Reilly MK, Robles O, Schwarz JB, Shakhmin AA, Shunatona HP, Sreenivasan R, Tivitmahaisoon P, Xu M, Zaw T, Wustrow DJ, Zibinsky M.
J Med Chem (2022) 65 : 12895 -12924
PubMed 36127295 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 36 7.07 8.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5200118 1 8.05
CHEMBL5190023 1 7.80
CHEMBL5170361 1 7.80
CHEMBL5179922 1 7.70
CHEMBL5199369 1 7.68
CHEMBL5172690 1 7.60
CHEMBL5180017 1 7.60
CHEMBL5193210 1 7.54
CHEMBL5208600 1 7.48
CHEMBL5196751 1 7.46
CHEMBL5197413 1 7.07
CHEMBL5199088 1 6.97
CHEMBL5183990 1 6.81
CHEMBL5200952 1 6.80
CHEMBL5196192 1 6.74
CHEMBL5201930 1 6.62
CHEMBL5175719 1 6.58
CHEMBL5189317 1 6.42
CHEMBL5195519 1 6.26
CHEMBL5202342 1 6.21
CHEMBL5172627 1 6.20
CHEMBL5180106 1 6.12
CHEMBL5204720 1 -
CHEMBL5201758 1 -
CHEMBL5188650 1 -
CHEMBL5194718 1 -
CHEMBL5192014 1 -
CHEMBL5177614 1 -
CHEMBL5208898 1 -
CHEMBL5176674 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5200118 IC50 = 9.0 nM 8.05
CHEMBL5190023 IC50 = 16.0 nM 7.80
CHEMBL5170361 IC50 = 16.0 nM 7.80
CHEMBL5179922 IC50 = 20.0 nM 7.70
CHEMBL5199369 IC50 = 21.0 nM 7.68
CHEMBL5172690 IC50 = 25.0 nM 7.60
CHEMBL5180017 IC50 = 25.0 nM 7.60
CHEMBL5193210 IC50 = 29.0 nM 7.54
CHEMBL5208600 IC50 = 33.0 nM 7.48
CHEMBL5196751 IC50 = 35.0 nM 7.46
CHEMBL5197413 IC50 = 86.0 nM 7.07
CHEMBL5199088 IC50 = 106.0 nM 6.97
CHEMBL5183990 IC50 = 156.0 nM 6.81
CHEMBL5200952 IC50 = 159.0 nM 6.80
CHEMBL5196192 IC50 = 183.0 nM 6.74
CHEMBL5201930 IC50 = 240.0 nM 6.62
CHEMBL5175719 IC50 = 262.0 nM 6.58
CHEMBL5189317 IC50 = 378.0 nM 6.42
CHEMBL5195519 IC50 = 545.0 nM 6.26
CHEMBL5202342 IC50 = 610.0 nM 6.21
CHEMBL5172627 IC50 = 628.0 nM 6.20
CHEMBL5180106 IC50 = 763.0 nM 6.12
CHEMBL5189372 IC50 - -
CHEMBL5195933 IC50 - -
CHEMBL5180483 IC50 - -
CHEMBL5194671 IC50 - -
CHEMBL5205311 IC50 - -
CHEMBL5207062 IC50 - -
CHEMBL5176674 IC50 - -
CHEMBL5208898 IC50 - -
CHEMBL5177614 IC50 - -
CHEMBL5192014 IC50 - -
CHEMBL5194718 IC50 - -
CHEMBL5188650 IC50 - -
CHEMBL5201758 IC50 - -
CHEMBL5204720 IC50 - -