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Assay Detail

CHEMBL5116677

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Protac activity at VHL/EGFR L858R/T790M mutant in human NCI-H1975 cells assessed as induction of degradation of EGFR incubated for 24 hrs in presence of afatinib by immunoblot analysis
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type NCI-H1975
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

von Hippel-Lindau disease tumor suppressor/EGFR (CHEMBL4523998)
Type PROTEIN-PROTEIN INTERACTION
Organism Homo sapiens

Publication

Discovery of highly potent and selective CRBN-recruiting EGFR<sup>L858R/T790M</sup> degraders in vivo.
Zhang W, Li P, Sun S, Jia C, Yang N, Zhuang X, Zheng Z, Li S.
Eur J Med Chem (2022) 238 : 114509 -114509
PubMed 35691176 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 1 6.67 6.67

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5175569 1 6.67

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5175569 EC50 = 215.8 nM 6.67