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Assay Detail

CHEMBL5126488

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at glucocorticoid receptor in rat INS-1 832/13 cells transfected with CCL2-promoter luciferase plasmid construct assessed as reduction in IL-1 beta induced inflammation by luciferase reporter gene assay
20
Total Activities
18
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Cell Type INS-1 832/13
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Glucocorticoid receptor (CHEMBL3368)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Stereoisomers of an Aryl Pyrazole Glucocorticoid Receptor Agonist Scaffold Elicit Differing Anti-inflammatory Responses.
Lato AM, Burke SJ, Ducote MP, Kennedy BJ, Collier JJ, Campagna SR.
ACS Med Chem Lett (2022) 13 : 1493 -1499
PubMed 36105346 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 7.42 9.50
%max 2 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5196090 1 9.50
CHEMBL5181366 1 9.20
CHEMBL384467 DEXAMETHASONE 4.0 2 8.90
CHEMBL5199091 1 8.30
CHEMBL5200995 1 7.80
CHEMBL5196115 1 7.70
CHEMBL81591 1 7.70
CHEMBL5183169 1 7.60
CHEMBL5194382 1 7.50
CHEMBL5207591 1 7.50
CHEMBL5185246 1 7.50
CHEMBL420767 1 7.20
CHEMBL5197908 1 6.80
CHEMBL389621 HYDROCORTISONE 4.0 2 6.50
CHEMBL5171084 1 6.40
CHEMBL5186941 1 6.40
CHEMBL5173367 1 5.70
CHEMBL5191812 1 5.40

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5196090 IC50 = 0.3162 nM 9.50
CHEMBL5181366 IC50 = 0.631 nM 9.20
CHEMBL384467 DEXAMETHASONE IC50 = 1.259 nM 8.90
CHEMBL5199091 IC50 = 5.012 nM 8.30
CHEMBL5200995 IC50 = 15.85 nM 7.80
CHEMBL5196115 IC50 = 19.95 nM 7.70
CHEMBL81591 IC50 = 19.95 nM 7.70
CHEMBL5183169 IC50 = 25.12 nM 7.60
CHEMBL5207591 IC50 = 31.62 nM 7.50
CHEMBL5185246 IC50 = 31.62 nM 7.50
CHEMBL5194382 IC50 = 31.62 nM 7.50
CHEMBL420767 IC50 = 63.1 nM 7.20
CHEMBL5197908 IC50 = 158.49 nM 6.80
CHEMBL389621 HYDROCORTISONE IC50 = 316.23 nM 6.50
CHEMBL5186941 IC50 = 398.11 nM 6.40
CHEMBL5171084 IC50 = 398.11 nM 6.40
CHEMBL5173367 IC50 = 1995.26 nM 5.70
CHEMBL5191812 IC50 = 3981.07 nM 5.40
CHEMBL389621 HYDROCORTISONE %max = 100.0 % -
CHEMBL384467 DEXAMETHASONE %max = 100.0 % -