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Assay Detail

CHEMBL5129286

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human TRPC5 channel expressed in HEK293 cells assessed as inhibition of EA-evoked Calcium influx by measuring reduction in intracellular Ca2+ level and measured upto 360 sec by Fluo-4/AM dye based FLIPR assay
36
Total Activities
36
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Short transient receptor potential channel 5 (CHEMBL1250411)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis of AC1903 analogs as potent transient receptor potential canonical channel 4/5 inhibitors and biological evaluation.
Chen L, Zhang Z, Tian H, Jiang S, Ji Y, Liu M, Shen J, Cao Z, Wang K.
Bioorg Med Chem (2022) 68 : 116853 -116853
PubMed 35653869 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 36 5.71 6.27

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5207902 1 6.27
CHEMBL5201191 1 6.24
CHEMBL5190035 1 6.17
CHEMBL5169865 1 6.09
CHEMBL5203265 1 6.04
CHEMBL5209321 1 6.02
CHEMBL5186222 1 5.99
CHEMBL5201859 1 5.87
CHEMBL5173485 1 5.87
CHEMBL5183590 1 5.74
CHEMBL5187515 1 5.66
CHEMBL5192702 1 5.66
CHEMBL5171565 1 5.65
CHEMBL5194092 1 5.64
CHEMBL5206434 1 5.64
CHEMBL5202944 1 5.62
CHEMBL5182312 1 5.58
CHEMBL5202586 1 5.53
CHEMBL5194160 1 5.53
CHEMBL5176140 1 5.50
CHEMBL5185813 1 5.47
CHEMBL5191462 1 5.33
CHEMBL1345058 1 4.19
CHEMBL5191534 1 -
CHEMBL5186146 1 -
CHEMBL5185490 1 -
CHEMBL5173272 1 -
CHEMBL5172936 1 -
CHEMBL5170000 1 -
CHEMBL5195015 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5207902 IC50 = 540.0 nM 6.27
CHEMBL5201191 IC50 = 570.0 nM 6.24
CHEMBL5190035 IC50 = 670.0 nM 6.17
CHEMBL5169865 IC50 = 820.0 nM 6.09
CHEMBL5203265 IC50 = 920.0 nM 6.04
CHEMBL5209321 IC50 = 960.0 nM 6.02
CHEMBL5186222 IC50 = 1020.0 nM 5.99
CHEMBL5201859 IC50 = 1340.0 nM 5.87
CHEMBL5173485 IC50 = 1350.0 nM 5.87
CHEMBL5183590 IC50 = 1820.0 nM 5.74
CHEMBL5187515 IC50 = 2210.0 nM 5.66
CHEMBL5192702 IC50 = 2210.0 nM 5.66
CHEMBL5171565 IC50 = 2240.0 nM 5.65
CHEMBL5194092 IC50 = 2270.0 nM 5.64
CHEMBL5206434 IC50 = 2300.0 nM 5.64
CHEMBL5202944 IC50 = 2410.0 nM 5.62
CHEMBL5182312 IC50 = 2640.0 nM 5.58
CHEMBL5194160 IC50 = 2930.0 nM 5.53
CHEMBL5202586 IC50 = 2950.0 nM 5.53
CHEMBL5176140 IC50 = 3130.0 nM 5.50
CHEMBL5185813 IC50 = 3350.0 nM 5.47
CHEMBL5191462 IC50 = 4630.0 nM 5.33
CHEMBL1345058 IC50 = 64970.0 nM 4.19
CHEMBL5206422 IC50 > 10000.0 nM -
CHEMBL5169949 IC50 > 10000.0 nM -
CHEMBL5200061 IC50 > 10000.0 nM -
CHEMBL5208734 IC50 > 10000.0 nM -
CHEMBL5200070 IC50 > 10000.0 nM -
CHEMBL5182054 IC50 > 10000.0 nM -
CHEMBL5195015 IC50 > 10000.0 nM -
CHEMBL5170000 IC50 > 10000.0 nM -
CHEMBL5172936 IC50 > 10000.0 nM -
CHEMBL5173272 IC50 > 10000.0 nM -
CHEMBL5185490 IC50 > 10000.0 nM -
CHEMBL5186146 IC50 > 10000.0 nM -
CHEMBL5191534 IC50 > 10000.0 nM -