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Assay Detail

CHEMBL5134828

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of PKN1 (unknown origin) by TR-FRET-based tracer displacement assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase N1 (CHEMBL3384)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development of dihydropyrrolopyridinone-based PKN2/PRK2 chemical tools to enable drug discovery.
Scott F, Fala AM, Takarada JE, Ficu MP, Pennicott LE, Reuillon TD, Couñago RM, Massirer KB, Elkins JM, Ward SE.
Bioorg Med Chem Lett (2022) 60 : 128588 -128588
PubMed 35104640 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 6.14 6.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5182936 1 6.92
CHEMBL250870 1 6.68
CHEMBL225519 1 6.52
CHEMBL250871 1 6.36
CHEMBL5175087 1 6.28
CHEMBL5202568 1 5.89
CHEMBL5187643 1 5.74
CHEMBL5170263 1 5.52
CHEMBL252724 1 5.35
CHEMBL255031 1 -
CHEMBL5208324 1 -
CHEMBL254392 1 -
CHEMBL5182853 1 -
CHEMBL5176291 1 -
CHEMBL5187274 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5182936 IC50 = 120.0 nM 6.92
CHEMBL250870 IC50 = 210.0 nM 6.68
CHEMBL225519 IC50 = 300.0 nM 6.52
CHEMBL250871 IC50 = 440.0 nM 6.36
CHEMBL5175087 IC50 = 520.0 nM 6.28
CHEMBL5202568 IC50 = 1300.0 nM 5.89
CHEMBL5187643 IC50 = 1840.0 nM 5.74
CHEMBL5170263 IC50 = 3000.0 nM 5.52
CHEMBL252724 IC50 = 4500.0 nM 5.35
CHEMBL255031 IC50 > 5000.0 nM -
CHEMBL5208324 IC50 - -
CHEMBL254392 IC50 - -
CHEMBL5182853 IC50 > 5000.0 nM -
CHEMBL5176291 IC50 > 5000.0 nM -
CHEMBL5187274 IC50 > 5000.0 nM -