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Assay Detail

CHEMBL5136126

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]-dexamethasone from rat glucocorticoid receptor incubated overnight by liquid scintillation counting analysis
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Glucocorticoid receptor (CHEMBL3368)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Discovery of Apararenone (MT-3995) as a Highly Selective, Potent, and Novel Nonsteroidal Mineralocorticoid Receptor Antagonist.
Iijima T, Katoh M, Takedomi K, Yamamoto Y, Akatsuka H, Shirata N, Nishi A, Takakuwa M, Watanabe Y, Munakata H, Koyama N, Ikeda T, Iguchi T, Kato H, Kikkawa K, Kawaguchi T.
J Med Chem (2022) 65 : 8127 -8143
PubMed 35652647 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 22 4.43 4.71

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5197264 1 4.71
CHEMBL5207249 1 4.65
CHEMBL5207123 1 4.59
CHEMBL5191961 1 4.54
CHEMBL5181002 1 4.54
CHEMBL5186524 1 4.42
CHEMBL5201004 1 4.40
CHEMBL5184647 1 4.38
CHEMBL5181278 1 4.37
CHEMBL5172350 1 4.35
CHEMBL5176336 1 4.33
CHEMBL5171957 1 4.26
CHEMBL2181929 MT-3995 2.0 1 4.08
CHEMBL5182687 1 -
CHEMBL5186231 1 -
CHEMBL5173089 1 -
CHEMBL5208644 1 -
CHEMBL5182675 1 -
CHEMBL5204612 1 -
CHEMBL5174401 1 -
CHEMBL5183454 1 -
CHEMBL5187656 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5197264 Ki = 19600.0 nM 4.71
CHEMBL5207249 Ki = 22200.0 nM 4.65
CHEMBL5207123 Ki = 25400.0 nM 4.59
CHEMBL5191961 Ki = 29000.0 nM 4.54
CHEMBL5181002 Ki = 29100.0 nM 4.54
CHEMBL5186524 Ki = 37900.0 nM 4.42
CHEMBL5201004 Ki = 40000.0 nM 4.40
CHEMBL5184647 Ki = 41500.0 nM 4.38
CHEMBL5181278 Ki = 43200.0 nM 4.37
CHEMBL5172350 Ki = 45000.0 nM 4.35
CHEMBL5176336 Ki = 47200.0 nM 4.33
CHEMBL5171957 Ki = 55200.0 nM 4.26
CHEMBL2181929 MT-3995 Ki = 84200.0 nM 4.08
CHEMBL5182687 Ki > 100000.0 nM -
CHEMBL5186231 Ki > 100000.0 nM -
CHEMBL5173089 Ki > 100000.0 nM -
CHEMBL5208644 Ki > 100000.0 nM -
CHEMBL5182675 Ki > 100000.0 nM -
CHEMBL5204612 Ki > 100000.0 nM -
CHEMBL5174401 Ki = 136000.0 nM -
CHEMBL5183454 Ki > 100000.0 nM -
CHEMBL5187656 Ki > 100000.0 nM -