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Assay Detail

CHEMBL5137593

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of C-terminal His6-tagged full length SARS CoV-2 3CL protease expressed in Escherichia coli BL21 (DE3) cells using Thr-Ser-Ala-Val-Leu-Gln-pNA substrate preincubated for 30 mins followed by substrate addition by microplate reader analysis
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Severe acute respiratory syndrome coronavirus 2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Replicase polyprotein 1ab (CHEMBL4523582)
Type SINGLE PROTEIN
Organism Severe acute respiratory syndrome coronavirus 2

Publication

Targeting SARS-CoV-2 Proteases and Polymerase for COVID-19 Treatment: State of the Art and Future Opportunities.
Cannalire R, Cerchia C, Beccari AR, Di Leva FS, Summa V.
J Med Chem (2022) 65 : 2716 -2746
PubMed 33186044 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 6.47 6.47

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL8260 BAICALEIN 2.0 1 6.47

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL8260 BAICALEIN IC50 = 340.0 nM 6.47