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Assay Detail

CHEMBL5142248

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of TDO (unknown origin) assessed as reduction in N-formylkynurenine formation using L-tryptophan as substrate incubated for 75 mins by UV absorbance based analysis
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tryptophan 2,3-dioxygenase (CHEMBL2140)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of 1-(Hetero)aryl-β-carboline Derivatives as IDO1/TDO Dual Inhibitors with Antidepressant Activity.
Zhang Y, Li Y, Chen X, Chen X, Chen C, Wang L, Dong X, Wang G, Gu R, Li F, Han F, Chen D.
J Med Chem (2022) 65 : 11214 -11228
PubMed 35938398 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 5.33 6.46

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL355606 1 6.46
CHEMBL39183 1 6.09
CHEMBL274452 1 5.94
CHEMBL5176579 1 5.94
CHEMBL275224 NORHARMANE 1 5.92
CHEMBL5186390 1 5.67
CHEMBL5196062 1 5.27
CHEMBL12014 HARMAN 1 5.20
CHEMBL3975325 1 5.14
CHEMBL5187479 1 5.08
CHEMBL38596 1 4.96
CHEMBL5195626 1 4.96
CHEMBL5194494 1 4.92
CHEMBL5180636 1 4.87
CHEMBL5184595 1 4.81
CHEMBL5188549 1 4.71
CHEMBL5172850 1 4.69

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL355606 IC50 = 350.0 nM 6.46
CHEMBL39183 IC50 = 810.0 nM 6.09
CHEMBL274452 IC50 = 1150.0 nM 5.94
CHEMBL5176579 IC50 = 1140.0 nM 5.94
CHEMBL275224 NORHARMANE IC50 = 1200.0 nM 5.92
CHEMBL5186390 IC50 = 2140.0 nM 5.67
CHEMBL5196062 IC50 = 5390.0 nM 5.27
CHEMBL12014 HARMAN IC50 = 6310.0 nM 5.20
CHEMBL3975325 IC50 = 7240.0 nM 5.14
CHEMBL5187479 IC50 = 8290.0 nM 5.08
CHEMBL38596 IC50 = 10840.0 nM 4.96
CHEMBL5195626 IC50 = 10920.0 nM 4.96
CHEMBL5194494 IC50 = 11980.0 nM 4.92
CHEMBL5180636 IC50 = 13390.0 nM 4.87
CHEMBL5184595 IC50 = 15330.0 nM 4.81
CHEMBL5188549 IC50 = 19510.0 nM 4.71
CHEMBL5172850 IC50 = 20290.0 nM 4.69