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Assay Detail

CHEMBL5158147

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human URAT1 mediated 14C-uric acid uptake expressed in HEK293 cells using 14C-uric acid as substrate incubated for 30 mins by liquid scintillation counter
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Solute carrier family 22 member 12 (CHEMBL6120)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Novel Bicyclic Imidazolopyridine-Containing Human Urate Transporter 1 Inhibitors as Hypouricemic Drug Candidates with Improved Efficacy and Favorable Druggability.
Zhao T, Zhang J, Tao Y, Liao H, Zhao F, Liang R, Shi X, Zhang Z, Ji J, Wu T, Pang J, Liu X, Zhan P.
J Med Chem (2022) 65 : 4218 -4237
PubMed 35084182 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 5.49 5.87

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5199889 1 5.87
CHEMBL5175070 1 5.60
CHEMBL5175653 1 5.59
CHEMBL5200839 1 5.51
CHEMBL5200105 1 5.45
CHEMBL5191410 1 5.43
CHEMBL5196337 1 5.43
CHEMBL5179331 1 5.31
CHEMBL2105720 LESINURAD 4.0 1 5.26

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5199889 IC50 = 1360.0 nM 5.87
CHEMBL5175070 IC50 = 2490.0 nM 5.60
CHEMBL5175653 IC50 = 2570.0 nM 5.59
CHEMBL5200839 IC50 = 3060.0 nM 5.51
CHEMBL5200105 IC50 = 3550.0 nM 5.45
CHEMBL5191410 IC50 = 3700.0 nM 5.43
CHEMBL5196337 IC50 = 3670.0 nM 5.43
CHEMBL5179331 IC50 = 4940.0 nM 5.31
CHEMBL2105720 LESINURAD IC50 = 5540.0 nM 5.26