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Assay Detail

CHEMBL5164050

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H](+)-pentazocine from human sigma 1 receptor transfected in human HEK293T cells assessed as inhibition constant by radioligand competition binding assay
30
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293T
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sigma non-opioid intracellular receptor 1 (CHEMBL287)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Preparation of novel analogs of 2-arylpiperidines and evaluation of their sigma receptor binding affinities.
Walby GD, Martin SF.
Eur J Med Chem (2022) 235 : 114310 -114310
PubMed 35395511 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 30 7.64 8.42

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5208940 2 8.42
CHEMBL5191108 2 8.35
CHEMBL5183680 2 8.32
CHEMBL5197224 2 8.27
CHEMBL5206383 2 8.17
CHEMBL5177703 2 8.10
CHEMBL5185602 2 7.97
CHEMBL5189334 2 7.82
CHEMBL5207336 2 7.75
CHEMBL5175530 2 7.54
CHEMBL5170591 2 7.30
CHEMBL5184025 2 6.98
CHEMBL5189037 2 6.80
CHEMBL5182001 2 6.61
CHEMBL5181506 2 6.22

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5208940 Ki = 3.8 nM 8.42
CHEMBL5208940 Ki = 3.802 nM 8.42
CHEMBL5191108 Ki = 4.5 nM 8.35
CHEMBL5191108 Ki = 4.467 nM 8.35
CHEMBL5183680 Ki = 4.8 nM 8.32
CHEMBL5183680 Ki = 4.786 nM 8.32
CHEMBL5197224 Ki = 5.4 nM 8.27
CHEMBL5197224 Ki = 5.37 nM 8.27
CHEMBL5206383 Ki = 6.7 nM 8.17
CHEMBL5206383 Ki = 6.918 nM 8.16
CHEMBL5177703 Ki = 8.0 nM 8.10
CHEMBL5177703 Ki = 7.943 nM 8.10
CHEMBL5185602 Ki = 10.72 nM 7.97
CHEMBL5185602 Ki = 11.0 nM 7.96
CHEMBL5189334 Ki = 15.0 nM 7.82
CHEMBL5189334 Ki = 16.22 nM 7.79
CHEMBL5207336 Ki = 18.0 nM 7.75
CHEMBL5207336 Ki = 18.2 nM 7.74
CHEMBL5175530 Ki = 29.0 nM 7.54
CHEMBL5175530 Ki = 29.51 nM 7.53
CHEMBL5170591 Ki = 50.0 nM 7.30
CHEMBL5170591 Ki = 50.12 nM 7.30
CHEMBL5184025 Ki = 104.0 nM 6.98
CHEMBL5184025 Ki = 104.71 nM 6.98
CHEMBL5189037 Ki = 160.0 nM 6.80
CHEMBL5189037 Ki = 158.49 nM 6.80
CHEMBL5182001 Ki = 245.47 nM 6.61
CHEMBL5182001 Ki = 245.0 nM 6.61
CHEMBL5181506 Ki = 602.56 nM 6.22
CHEMBL5181506 Ki = 609.0 nM 6.21